摘要
目的建立冠心宁胶囊中芍药苷含量测定方法。方法:采用RP-HPLC法。固定相为Waters C18(3.9mm×300mm,10μm)色谱柱,流动相:乙腈-水(1:4),检测波长为230nm,流速为1.0mL·min^-1,柱温为25℃。结果芍药苷的平均回收率为100.58%(RSD=1.45%,n=5),线性范围为0.43~2.18μg(r=0.9999)。结论本法快速,准确,灵敏,简便易行,可用于冠心宁胶囊的质量控制和评价。
OBJECTIVE To establish a determination method for Paeoniflorin in Guanxinning capsules. METHODS Using HPLC with Waters C18 (3.9 mm×300 mm, 10 μm) column, acetonitrile-H2O (1 : 4) served as the mobile phase with the flow rate of 1.0 mL · min^-1. The column temperature was 25 ℃ and the detection wavelength was set at 230 nm. RESULTS The average recovery was 100. 58% (n= 5, RSD=1.45 % ) . The standard curve was linear in the concentration range of 0. 43-2.18 μg (r=0. 999 9). CONCLUSIONS The method is simple, rapid, accurate and reliable, and provides a method for the quality control and evaluation of Guanxinning capsules.
出处
《中南药学》
CAS
2006年第1期24-25,共2页
Central South Pharmacy