摘要
目的:探讨糖毒清治疗糖尿病肾病肾功能不全的可能机制。方法:采用5/6肾切除合中等剂量的STZ腹腔注射方法建立糖尿病肾病肾功能不全模型,将造模成功的大鼠50只随机分为5组:模型组(生理盐水2mL/d灌胃)、糖毒清高剂量组[糖毒清6.75g/(kg·d)灌胃];糖毒清中剂量组[糖毒清4.5g/(kg·d)灌胃]、糖毒清低剂量组[糖毒清2.25g/(kg·d)灌胃]、尿毒清组[尿毒清2.25g/(kg·d)灌胃]。另外10只正常对照组大鼠予生理盐水2mL/d灌胃;治疗10周后,通过免疫组化的方法检测各组大鼠肾组织的VCAM-1表达情况。结果:模型组大鼠肾组织VCAM-1表达明显增多,而糖毒清组大鼠肾组织VCAM-1的表达明显减少。结论:糖毒清可以减少糖尿病肾病肾功能不全大鼠肾组织VCAM-1的过度表达,从而达到控制或延缓糖尿病肾病肾功能恶化的进程。
Objective： To clarify the possible mechanisms of Tangduqing granules through examining and eompanng me expressions of VCAM-1 in renal tissues of model rats with diabetic renal insufficiency （DRI）. Methods： Sixty male SD rats were experienced combination of 5/6 nephrectomy and abdominal cavity injection with midium-dose streptozotocin to establish model rats with DRI. Fifty successful model rats with DR/were divided into five groups randomly： Model Group （Administered with NS 2mL/d）,High Dose Group [Administered with Tangduqing 6.75g/ （kg.d）],Medium Dose Group [Administered with Tangduqing 4,5g/ （kg.d）],Low Dose Group [Administered with Tangduqing 2,25g/ （kg.d）], Niaoduqing Group [Administered with Niaoduqing 2,25g/ （kg.d）]. Ten age-matched healthy male SD rats were served as controls （Administered with NS 2mL/d）. Ten weeks later, the expressions of VCAM- 1 in renal tissues of rats with DR/were observed through immunohistochemical method.Results： The expressions of VCAM-1 in renal tissues of rats with DR/were obviously enhanced. The expressions of VCAM-I in renal tissues of rats with Tangduqing were obviously decreased. Conclusion ：Tangduqing might reduce the over-expression of VCAM-I in renal tissues of rats with DRI, and control or delay the development of renal failure.
出处
《实用中西医结合临床》
2006年第1期1-2,共2页
Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine
基金
广东省科技厅(2002C30502)
广东省教育厅(Z02010)资助项目
关键词
糖尿痛肾痛
肾功能不全
VCAM-1
糖毒清
实验研究
益气助阳
化瘀泄浊
Diabetic Renal Insufficieney
VCAM-1
Tangduqing
empideal study
removing blood stasis and resolving masses
supplementing qi and aiding yang
