摘要
目的探讨油酸性急性呼吸窘迫综合征(ARDS)beagle犬血浆及肺泡灌洗液血管内皮生长因子(VEGF)、可溶性细胞间黏附分子-1(ICAM-1)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)水平的改变。方法12只英国纯种beagle犬,静脉注射油酸0.15 mL/kg,在注射油酸前、后1 h,出现ARDS的典型表现时,抽血测VEGF、sICAM-1、IL-8、TNF-α,并对此时相作肺泡灌洗液VEGF、sICAM-1、IL-8、TNF-α的测定。结果beagle犬静脉注射油酸后1 h血浆TNF-α升高(P<0.05),血浆及肺泡灌洗液IL-8s、ICAM-1和VEGF在1 h较油酸前没有明显变化(P>0.05),beagle犬油酸型ARDS模型建立后血浆及肺泡灌洗液VEGF、sICAM-1、IL-8、TNF-α均显著高于建模前(P<0.05)。结论VEGFs、ICAM-1、IL-8、TNF-α在beagle犬油酸型ARDS发生发展过程中可能均起重要作用,其水平的高低可能与ARDS严重程度及预后有关。
Objective To observe changes of vascular endothelial growth factor （VEGF）, soluble intercellular adhesion roolecule - 1 （slCAM - 1）, interleukin- 8（ IL- 8） ,TNF- α in plasma and bronchoalveolar lavage fluid （BALF） in beagle dogs with oleic acid - induced ARDS. Methods 12 beagle （togs were infused with oleic acid（OA）. Levels of TNF-α and IL- 8 in plasroa and BALF were tested by y（RIA）, levels of slCAM - I and VEGF by EHSA. Results Levels of TNF - α in plasma and BALF markedly increased at 1 hour after the infusion of oteic acid （ P 〈 0.05 ）, level of VEGF, sICAM - 1 and IL - 8 didn＇ t ehange at 1 hour. The concentrations of sICAM- 1,VEGF, IL 8 and TNF- α in plasma and BALF in beagle dogs with oleic acid- induced ARDS were higher than those before OA injection （ P 〈 0.05）. Conclusions Levels of VEGF, sICAM- 1, IL- 8, TNF- α are higher than those before OA injection. They may play an important role in beagle dogs with oleic acidinduced ARDS, and serve as a index to estimate ARDS degree and clinical prognosis.
出处
《中国急救医学》
CAS
CSCD
北大核心
2006年第2期118-119,共2页
Chinese Journal of Critical Care Medicine
基金
重庆市自然科学基金资助项目(渝科发计字[2003]20号)
关键词
呼吸窘迫综合征
比格犬
血管内皮生长因子
可溶性黏附分子-1
白细胞介素-8
肿瘤坏死因子-α
Acute respiratory distress syndrome（ARDS）
Beagle dogs
Vascular endothelial growth factor（VEGF）
Soluble intercellular adhesion molecule - 1（ sICAM - 1 ）
Interleukin（ IL- 8）
