摘要
用食蟹猴疟原虫Ｂ株早期环状体接种１只恒河猴，接种后第２－１２天（血内仅存在１群原虫）测试了由第１－４代裂殖子形成的４代配子体（Ｇ）对大劣按蚊（海南株）感染性的活力周期。结果表明第１－４代裂殖子均形成了一定数量的Ｇ，由裂殖子发育到Ｇ功能成熟（可使蚊感染）各代有差异，第２代Ｇ至少需５５±１ｈ，第３代Ｇ则需６５±１ｈ；发育至７２±４ｈ时活力最旺，Ｇ寿命长者可达１００ｈ；生活期（对蚊有感染力的时间）第１、２代Ｇ约为１２－４２ｈ，第３代Ｇ约为１０－１９ｈ。Ｇ密度与蚊胃卵囊均数不成正比，第１、２代Ｇ对蚊的感染性比第３。
This paper reports the results of feeding batches of Anopheles dirus (Hainan strain) successively on a monkey infected with small ring stage of plasmodium cynomolgi bastianellii. 40 batches of mosquitoes were fed on the monkey from 2nd-12th day after parasite inoculation. 10 to 30 mosquitoes from each batch were dissectced on the 6th or 7th day after infection and the oocysts were counted. The infection rate of mosquitoes and the mean oocyst count per infected mosquito were taken as the criteria to evaluate the viability of the gametocytes. The blood films were taken when mosquitoes fed, and the number of asexual, schizont parasites and the mature gametocytes were counted by routine method. The conclusions drawn from this work are: (1)The development time required from merozoites to functional mature gametocytes would be approximately 55±1 hours in the second generation and 65±1 hours in the third generation. (2)The longest life-span of the gametocytes could be over 100 hours. (3)The infectivity of gametocytes to mosquitoes reached the highest level during the late stage of trophozoites of the next generation (i.e.24±4 hours after the schizogony). (4)The infective stage (being infective to mosquito) of gametocytes remained as long as 12-42 hours in the second generation and 10-19 hours in the third generation. (5)There is no marked correlation between the gametocyte density and the mean oocyst count. The infectivity of gametocytes of the first or second generation to mosquitoes was better than that of the third or fourth generation.
出处
《寄生虫与医学昆虫学报》
CAS
1996年第2期71-77,共7页
Acta Parasitologica et Medica Entomologica Sinica
基金
国家自然科学基金资助项目NO.39170693
关键词
食蟹猴疟原虫
B株
配子体
大劣按蚊
感染
Plasmodium cynomolgi bastianellii Infective stage of gametocytes Anopheles dirus (Hainan strain).
