摘要
从７例阴道炎患者阴道分泌物分离出７株细菌，均具有相同的生物学特性，Ｇ－梭状菌，单、成对或链状。无荚膜，无芽抱，无鞭毛。兼性厌氧菌，在大气中培养不生长，在５％～１０％ＣＯ２中或烛缸培养１８～２４ｈ才能形成菌落，ＭａｃＣｏｎｋｅｙ培养基不生长，最适生长温度３５℃－３７℃。氧化酶阴性，接触酶阴性，不还原硝酸盐，发酵糖类（指示剂用溴甲酚紫），克氏双糖铁高层和斜面均发生产酸反应，七叶苷水解试验阳性，马尿酸盐水解试验阴性。经ＢｉｏｌｏｇＭｉｃｒｏｓｔａｔｉｏｎＳｙｓｔｅｍ自动化细菌鉴定系统检测无确定结果，ＤＮＡＧ＋Ｃ含量为４２．３ｍｏｌ％、１６ｓｒＲＮＡ序列测定结果经计算机检索国际基因菌库ＥＭＢＬ和ＧｅｎＢａｎｋ所有序列进行比较，表明该白与已知科、属亲缘关系较远，结合其表型特征，建议建立新属，命名为吉林链梭菌（Ｓｔｒｅｐｔｏｆｕｓｉａｇｅｎ．ｎｏｖ．Ｊｉｌｉｎａｓｐ．ｎｏｖ．）该菌已收藏在中国微生物菌种保藏中心ＣＧＭＣＣＮＯ．０２１５Ｔ（Ｔ＝ｔｙｐｅｓｔｒａｉｎ）．该菌１６ＳｒＲＮＡ片断已被国际基因库收录，接收号为Ｕ３４３６５。
Seven bacterial strains isolated from the secretion of seven vaginitis patients. These strains were same physiological characteristics identified as gram--negative short rods in singles pairs and chains. By electron micrographs its cells showed fusiform. Capsules. spored and flagella were not present. CO2 (5% - 10%) was required for the growth of all strains on blood agar. All strains can not grow on MacConkey's media. The optimum temperature for growth was 35℃ ～ 37℃. Oxidase and catalase test was negative. Glucose was fermented with production of acid but no gas (Indicator was bromcresol purple). Acid was produced form slant and butt Kligler iron agar. Nitrates were not reduced to nitrites Esculin was hydrolyzed, but hippurate was not hydrolyzed. The mol% G+C of the DNA was 42.3 (Tin). On the basis of 16S rRNA oligonucleotide similarity, dendrogram and average linkage clustering, these new bacterial strains can not fit well into any of the currently recognized categories. Therefore, a new genus, Streptofusia. gen. nov. jilina. sp. nov was proposed 16S rRNA has been provided GenBank accession numbers (BankItl2569. U34365). The type strain is CGMCC NO. 021 5T (T = type strain)
出处
《微生物学通报》
CAS
CSCD
北大核心
1996年第4期195-199,共5页
Microbiology China
关键词
细菌
吉林链梭菌
新菌
分类学
Strepofusia. gen. nov. jilina. sp. nov, 16S rRNA, Identification, Taxonomy
