摘要
于国内外首次报道采用改良兔血胰(月示)琼脂培养基,从狐体及其流产胎儿脏器中,分离得到阴道加德纳氏菌共145株。其中从流产胎儿脏器分离得到26株,分离率为92.86％。从流产空怀狐阴道分泌物中分离得到118株,分离率34.01％。从阳性取皮狐鼠蹊淋巴结分离得到1株,分离率2％。血液中未分离到本菌。通过对病原菌的分离,揭示了该菌在狐体内的存在部位,优选出最佳的分离途径、时机与方法。人工感染试验结果证实了本菌的致病性。
It is the first time that this paper reports on using improved rabbit blood agar culture medium to isolate 145 strains Gardnerella vaginalis from the foxes and their abortion fortus organs of main fox farms. Among the strains, 26 strains were isolated from the abortion foetus organs, isolation rate was 92. 86%; 118 strains were isolated from the vaginal exeretion of the abortion and empty foxes, isolation rate was 34.01% ; 1 strain was isolated from inguinal lymph node of the pelted positive fox, isolation rate was 2%. None was isolated from blood.By causative agent isolation,we revealed the bacterium survival position in vivo,and the best isolation route isolation opportunity and isolation methods were selected. By artificial infection test, we have proved pathogenicity of the bacterium.
出处
《微生物学报》
CSCD
北大核心
1995年第1期28-32,共5页
Acta Microbiologica Sinica
基金
农业部畜牧业"八五"重点研究专题
关键词
狐狸
阴道加德纳氏菌
病原分离
人兽共患
感染
Fox
Gardnerella vaginalis
Causative agent isolation
Artificial infection
Zoonosis
