摘要
以高纯空气(O2∶N2=21∶79)为加压介质,研究了0.5Mpa压力下面包酵母CICC1447和CICC1339细胞生长及细胞内谷胱甘肽、麦角固醇的含量变化。结果表明加压培养时两株酵母菌的对数生长期延迟出现,对数生长期的持续时间缩短,而且两株菌的比生长速率均明显低于对照组,同时两株菌的倍增时间也较对照组有所延长;压力刺激可显著提高面包酵母细胞内谷胱甘肽(GSH)的含量,但麦角固醇含量的变化却不明显。在0.5MPa压力下保压培养3h时CICC1447胞内谷胱甘肽含量比常压对照组提高了42.6%,而加压3h后麦角固醇含量比对照组提高了20.1%;加压培养6h时CICC1339胞内谷胱甘肽含量较对照组提高了58.7%,但其麦角固醇含量反而降低。这说明不同的酵母菌对压力刺激的反应是不同的。
The growth changes of glutathione （GSH） and ergosterol in Saccharomyces cerevisiae （ CICC1447 and CICC1339） were detected under 0.5Mpa pressure with compressed high-pure air （O2：N2 =21： 79）. The results showed that logarithmic phases of the two strains were delayed ; their biomass and special growth rate were lower than those of control sample （0. 1MPa） and the double time were prolonged under 0.5MPa. High - pressure could increase the content of GSH obviously, compared to ambient atmosphere control samples. When the holding time was 3h, the content of GSH and ergosterol in CICC1447 increased 42.6% and 20. 1%, respectively. However, the content of GSH in CICC1339 increased 58.7% when the holding time was 6h, while ergosterol content reduced. The results indicated that different yeast strains have different stress-response mechanism to copy with high-pressure shock.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2006年第1期56-59,共4页
China Biotechnology
基金
国家自然科学基金资助项目(20176041)
关键词
高压
谷胱甘肽
麦角固醇
面包酵母
High pressure Glutathione Ergosterol Saccharomyces cerevisiae
