摘要
The chloroplast genes matK and rbcL, ribo- somal gene 18S and ITS regions of nuclear ribosomal DNA from Ephedra rhytidosperma, a species endemic to China, were sequenced and its phylogenetic position was investi- gated. Independent and combined phylogenetic analyses for the DNA sequences from 16 taxa representing 15 species of the genus Ephedra were performed using the maximum par- simony (MP), neighbor-joining (NJ), minimum evolution (ME) and maximum likelihood (ML) methods. The results indicate that E. rhytidosperma is closely related to E. equiset- ina. The divergence time between them is estimated to be 10.85±2.44 Ma based on the results of the relative-rate tests and the evolutionary rate of rbcL gene.
The chloroplast genes matK and rbcL, ribosomal gene 18S and ITS regions of nuclear ribosomal DNA from Ephedra rhytidosperma, a species endemic to China, were sequenced and its phylogenetic position was investigated. Independent and combined phylogenetic analyses for the DNA sequences from 16 taxa representing 15 species of the genus Ephedra were performed using the maximum parsimony （MP）, neighbor-joining （N J）, minimum evolution （ME） and maximum likelihood （ML） methods. The results indicate that E. rhytidosperma is closely related to E. equisetina. The divergence time between them is estimated to be 10.85±2.44 Ma based on the results of the relative-rate tests and the evolutionary rate of rbcL gene.
基金
supported by the Chinese National Key Project for Basic Research(“973"”)(2003CB715904)
the National Natural Science Foundation of China(Grant Nos.30230030&30370105).
关键词
麻黄属植物
中国
叶绿体
核糖体
DNA序列
系统发育学
Ephedra, Ephedra rhytidosperma, cpDNA matK gene, Chloroplast rbcL, Nuclear ribosomal 18S, nrDNA ITS regions, phylogenetic analysis
