摘要
A simple method for preparation of template DNA suitable for PCR amplification from herbarium samples and plant tissues rich in byproducts, e.g. polysaccha-rides, tannins, polyphenolic, and terpenoids compounds, is described. The total DNA from regular extraction procedure is absorbed by a small amount of glass powder and the final precipitation of glass powder is used directly as a template for PCR. Taking six plant taxa, including the herbarium specimens of Lythraceae collected from Namibia in 1957 and the silicon-dried leaf tissue from mangrove plants (Rhizo-phoraceae and Combretaceae) rich in by-products as examples, the PCR products, including nrDNA ITS regions and cpDNA rbcL gene, amplified following the regular and new methods respectively are compared. Our method provides a simple, rapid and economic approach to purify and prepare template DNA for PCR from special plant materials.
A simple method for preparation of template DNA suitable for PCR amplification from herbarium samples and plant tissues rich in byproducts, e.g. polysaccharides, tannins, polyphenolic, and terpenoids compounds, is described. The total DNA from regular extraction procedure is absorbed by a small amount of glass powder and the final precipitation of glass powder is used directly as a template for PCR. Taking six plant taxa, including the herbarium specimens of Lythraceae collected from Namibia in 1957 and the silicon-dried leaf tissue from mangrove plants (Rhizophoraceae and Combretaceae) rich in by-products as examples, the PCR products, including nrDNA ITS regions and cpDNA rbcL gene, amplified following the regular and new methods respectively are compared. Our method provides a simple, rapid and economic approach to purify and prepare template DNA for PCR from special plant materials.
基金
This work was supported by the Chinese National Science Funds for Distinguished Young Scholar (Grant No. 39825104)
the National Natural Science Foundation of China (Grant Nos. 39970057 and 30170071)
the Natural Science Foundation of Guangdong Provinc
