摘要
用粉纹夜蛾（Ｔｒｉｃｈｏｐｌｕｓｉａｎｉ）建立的一株细胞系ＢＴＩ－ＴＮ－ＳＢ１对苏云金杆菌库斯塔克变种（Ｂａｃｉｌｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓｖａｒｋｕｒｓｔａｋｉ，Ｈ３血清型）和蜡螟变种（Ｂｔ．ｖａｒ．ｇａｌｌｅｒｉａｅ，Ｈ５血清型）的晶体毒素进行了抗性选择。实验结果表明：ＢＴＩ－ＴＮ－５Ｂｌ细胞系对蜡螟变种的敏感性更高．进一步用Ｂｔ．－Ｈ５型晶体毒素对该细胞系进行抗性选择，经ＭＴＴ法测得连续处理细胞至第１５代时，抗性达到２．
A cell line of cabbage looper (Trichoplusia ni) BTI-TN-5Bl was used to select the resistance to Bacillus thuringiensis δ-endotoxin. Primary experiments indicated that the BTI-TN-5Bl cell line was more sensitive to Bt. var. galleriae (serotype H5) than to Bt. var. kurstaki (serotype H5). Further studies were made to select the resistance of 5Bl cells to Bt. -H5 δ-endotoxin. With a MTT-based assay, the fifteen generations of selection caused 2. 5-fold resistance of cabbage looper cell line compared with the the normal 5Bl cell line.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
1996年第1期77-80,共4页
Journal of Central China Normal University：Natural Sciences
基金
国家自然科学基金
