摘要
研究在体情况下迷走神经刺激(VNS)和乙酰胆碱(Ach)灌注对心房肌不同部位的电生理影响,并探讨其诱发心房颤动(AF)的机制。10只杂种犬自身随机对照,运用单相动作电位(MAP)记录技术,同步记录10只开胸犬的右心耳(RAA)、高位右房(HRA)、低位右房(LRA)、左心耳(LAA)、高位左房(HLA)、低位左房(LLA)的MAP,分别给予切断迷走神经、VNS、Ach灌注(分别做为对照组、VNS刺激组、Ach灌注组)后,观察诱发AF的情况和动作电位时程APD50、APD90和APD离散(dAPD)的变化。结果:10只犬在VNS刺激和Ach灌注同时,右心耳单一刺激分别有7只和6只犬诱发AF;VNS明显缩短APD50、APD90,其中RAA缩短最明显(APD50从72±5ms到19±4ms,APD90从136±7ms到43±5ms,P<0.001);Ach灌注也明显缩短APD50和APD90,与VNS相比,LLA的APD90缩短更明显(47±6msvs62±8ms,P<0.01);VNS明显升高心房肌APD50和APD90的离散(17±5msvs7±3ms,25±7msvs8±5ms,P<0.01)。结论:VNS和Ach灌注可引起APD缩短和离散升高,但影响的部位和程度稍有差异,都易诱发AF。
To investigate the electrophysiological effect of vagal nerve stimulation（VNS） and acetylcholine（Ach） on atrial myocardium in vivo and to discuss the mechanisms of atrial fibrillation（AF）, ten autocontrolled dogs were performed opening chest. With monophasic action potential （MAP） recording technique, right atrial appendage（RAA）, high right atrium （HRA）, low right atrium （LRA）, left atrial appendage （LAA）, high left atrium （HLA） , and low left atrium （LLA） were recorded by specially designed needle electrodes at the atrial myocardium. After cervical vagosympathetic was cutted, VNS and Ach were administrated respectively. At the same time, action potential duration（APD） , dispersion of APD （dAPD） and AF were observed. Results： During VNS and ACh perfusion, AF was evoked easily. APD5o and APD90 decreased significantly during VNS, especially in RAA（ APD5o from 72±5ms to 19 ± 4ms, APD90 from 136±7ms to 43 ±5ms,P 〈0.001 ）. During Ach perfusion, APD90 was shorter than that during VNS in LLA （ 47 ± 6ms vs 62 ± 8ms ,P 〈 0.01 ）. During VNS, dAPD increased significantly in APD5o and APD90 （ from 17 ± 5 ms to 7± 3 ms,from 25±7 ms to 8 ±5 ms, P 〈0.01 ）. Conclusion： VNS and Ach perfusion can shorten APD and increase dAPD, and induce AF easily.
出处
《中国心脏起搏与心电生理杂志》
2005年第6期462-464,共3页
Chinese Journal of Cardiac Pacing and Electrophysiology
基金
高等学校博士学科点专项科研基金心房颤动患者的重构现象及药物干预的基础研究
关键词
电生理学
乙酰胆碱
迷走神经
单相动作电位
心房肌
犬
Electrophysiology
Acetylcholine
Vagal nerve
Monaphasic action potential
Atrial myocardium
Canine
