摘要
目的:观察表皮细胞生长因子(EGF)、碱性成纤维生长因子(bFGF)体外诱导骨髓间充质干细胞(MSCs)分化为神经细胞后,神经元微管相关蛋白Tau和神经元特异性烯醇化酶(NSE)的表达。方法:取P4代MSCs分为EGF、bFGF和EGF+bFGF 及对照组;免疫细胞化学法检测Tau蛋白和NSE表达,ELISA法分析各组细胞Tau蛋白含量。结果:免疫细胞化学检测显示 EGF+bFGF组Tau蛋白和神经元特异性烯醇化酶NSE阳性细胞率最高,bFGF组、EGF组和对照组依序递减;各组Tau蛋白含量结果与Tau蛋白阳性细胞率一致。结论:EGF和bFGF均可促进MSCs向神经细胞分化,并表达Tau蛋白和NSE,二者具有协同作用。
Objective： To observe the expression of Tau protein and neuron-specific enolase （NSE） after differentiation of bonemarrow mesenchymal stem cells （MSCs） into neural cells induced by EGF and bFGF in vitro. Methods： P4-MSCs were divided into 4 groups; EGF＋bFGF, bFGF, EGF, and control groups. After 14-day induction, positive rates of Tan protein and NSE were tested by irnmunocytochemistry and Tan protein content assay was analyzed with ELISA test. Results： It was found that the positive rates of Tau protein and NSE in EGF＋ bFGF group were the highest, followed by bFGF, EGF and control groups in turn （P〈0. 001）, In eachgroup, the content of Tan protein was in consistent with its positive rate. （P〈0. 001）. Conclusion： EGF and bFGF can induce MSCs into neural cells in vitro and express Tan protein and NSE. They have synergistic effects during the inducing process.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2005年第6期615-617,640,共4页
Chinese Journal of Anatomy
基金
河南省科技攻关基金(0424410085)河南省医学科技创新人才工程项目(2004013)
