摘要
目的研究小鼠心肌上清液诱导小鼠骨髓瘤Sp2/0细胞凋亡的作用.方法在Sp2/0细胞培养孔中,分别加入不同稀释度(1∶6,1∶12,1∶24)的小鼠心肌上清液和环磷酰胺,24h后镜下观察细胞形态,并于48h后流式细胞仪检测凋亡细胞的比率;同时,Sp2/0细胞和小鼠心肌细胞共培养,10h后观察Sp2/0细胞形态学变化;并用不同稀释度(1∶5,1∶10,1∶20,1∶40)小鼠心肌裂解上清液、肝脏裂解上清液、胸腺裂解上清液及环磷酰胺诱导Sp2/0,HeLa,Hep-2和Vero细胞凋亡,并比较其差异.结果不同稀释度的心肌裂解上清液均可诱导Sp2/0细胞的凋亡,尤以高稀释度更明显;小鼠心肌细胞与Sp2/0细胞共培养10h后,少部分Sp2/0细胞出现凋亡;小鼠心肌裂解上清液、肝脏裂解上清液、胸腺裂解上清液均可引起Sp2/0,HeLa,Hep-2和Vero细胞发生凋亡,但小鼠心肌裂解上清液作用明显,且作用时间持久.结论小鼠心肌组织裂解上清液具有明显诱导小鼠骨髓瘤Sp2/0细胞凋亡的作用.
AIM： To study the roles of mouse cardiac muscle lysate supernatant in inducing the apoptosis of mouse myeloma Sp2/0 cells. METHODS： Various concentrations （1：6, 1：12, 1：24） of mouse cardiac muscle lysate supernatant and cyclophosphamide were added into the Sp2/0 cell culture wells. After 24 h, microscope was used to observe the morphological changes of Sp2/0 cells and apoptosis rate was determined by flow cytometry after 48 h. Morphological changes of Sp2/0 cells co-cultured with mouse cardiac muscle cells were observed after 10 h. The apoptosis of Sp2/0, Hela, Hep-2 and Vero cells was induced with various concentrations （ 1：5, 1 ： 10, 1：20 and 1：40） of mouse cardiac muscle lysate supernatant, liver lysate supernatant, thymus lysate supernatant and cyclophosphamide, and then the difference of apoptotic rate among them was compared. RESULTS： Mouse cardiac muscle lysate supernatant could induce the apoptosis of the Sp2/0 cells and the apoptotic rate increased in a concentration-dependent manner, but there was no difference between mouse cardiac muscle lysate supernatant group and cyclophosphamide group. After a 10-hour co-culture with mouse cardiac muscle cell lysate, the apoptosis of a few Sp2/0 cells was seen. The mouse cardiac muscle lysate supernatant, liver lysate supernatant and thymus lysate supernatant could all induce apoptosis of the Sp2/0, HeLa, Hep-2 and Vero cells. But the effect of the mouse cardiac muscle lysate supernatant was more obvious and permanent. CONCLUSION： The mouse cardiac muscle lysate supernatant can significantly induce apoptosis of mouse myeloma Sp2/0 cells.
出处
《第四军医大学学报》
北大核心
2005年第24期2245-2247,共3页
Journal of the Fourth Military Medical University
