摘要
目的:通过环磷酰胺诱导大鼠嵌合及免疫耐受的产生,并初步探讨其安全性和可行性。方法:将Lewis大鼠随机分为三组。A组于实验前一天腹腔注射150mg/kg环磷酰胺,第1天经尾静脉输注2×108个供鼠骨髓细胞;B组于实验前一天腹腔注射等渗盐水2mL,第1天经尾静脉输注2×108个供鼠骨髓细胞;C组于实验前一天腹腔注射150mg/kg环磷酰胺,第1天经尾静脉注射等渗盐水2mL。观察各组大鼠移植物抗宿主病(GVHD)的发生。于输注骨髓细胞后第14天及移植皮肤出现排斥反应时,取外周血检查供鼠来源T细胞占总T细胞的比例。于第15天取供鼠腹部皮肤,进行皮肤移植,观察各组移植物存活时间。结果:A组可观察到明显的GVHD,第14天和移植皮肤被排斥时,供鼠来源T细胞占总T细胞的比率分别为25.49%和0.06%。A组移植皮肤存活时间为29.3d,显著长于B组(8.7d)和C组(8.4d)。结论:环磷酰胺可诱导嵌合免疫耐受的产生,在嵌合状态下可检测到供鼠来源的T细胞。当移植皮肤被排斥时,供鼠来源T细胞占总T细胞的比例显著降低。
Objective： The study was designed to induce chimerism and immune tolerance by Cyclophsmide and the safety was evatuated. Methods： Lewis rats were divided into three groups. Group A received 150 mg/kg Cyclophsmide by intraperritoneal injection（ i. p. ） on day^-1 and 2 ×10^8 bone marrow cells from BN rats was transfused through tail vein on day 0. Group B received 2ml NS i. p. on day^-1 and then 2 ×10^8 bone marrow cells from BN rats through vein tail on day 0. Group C received 150mg/kg Cyclophsmide i.p. on d^-1 and then 2ml normal sodium（NS） through tail vein on d0. The symptom of graftversus-host disease （GVHD） was assessed. On day 14 and the day when grafted skin was rejected, chimerism was detected by flow cytometery. On day 15 all rats of the groups received skins from BN rats and the survival time of grafted skin was observed. Results ： GVHD was observed in Group A and two rats of this group died. Chimerism was observed in Group A but not in Group B and C. Survival time of grafted skin in Group A was significantly longer than in Group B and Group C. Conclusion： Chimerism and immune tolerance can be induced by Cyclophosphmide and when the grafted skin was rejected, the ratio of T cells from BN significantly decreased.
出处
《肠外与肠内营养》
CAS
2007年第2期83-86,90,共5页
Parenteral & Enteral Nutrition
基金
江苏省自然科学基金(BK2004426)
