摘要
用124个10碱基随机引物对烟草2对雄性不育系及其保持系线粒体DNA的RAPD分析表明,不育系与保持系间有112个引物未扩增出多态性片段,占引物总数的92.56%,这反映了不育系和保持系线粒体的同源性。同时,在不育系与保持系的线粒体基因组间也发现了明显的差异,这些差异片段与雄性不育的关系还需进一步研究。
The mitochondrial DNA of 2 CMS tobacco lines and their homolog maintainer lines were analysed by the method of random amplified polymorphic DNA using 124 random 10 - base primers. The result showed that 112 primers, 92.56% of total primers, did not amplified ploymorphic band between CMS and maintainer lines. This indicated the homolog of mitochondria between CMS and maintainer line. Meanwhile, some obvious differences in mitochondrial genome were detected between CMS and maintainer line. The relationship of these different bands in CMS to male sterility needs farther study.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2005年第6期826-830,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
云南省农业生物技术重点实验室开放项目基金(2003E01)
江西省烟叶科学研究所资助项目
