摘要
目的探讨复方抗纤二号抗肝纤维化的治疗机制。方法雄性Wistar大鼠分成5组,除正常对照外,余4组均腹腔注射猪血清(0.5 ml/次,2次/周,共12周)用作肝纤维化造模。抗纤二号早期治疗组B在第3周给予中药灌胃,1 ml/100 g体重,每天一次。抗纤二号晚期治疗组C在第9周给予中药灌胃,1 ml/100 g体重,每天一次。γ-干扰素治疗组D在第9周每天皮下注射10万单位的干扰素。模型组A和正常对照组N给等量的生理盐水灌胃。12周末杀大鼠,苏木精-伊红染色和Masson染色观察肝纤维化的形成,免疫组织化学观察平滑肌肌动蛋白(SMA)的表达。同时逆转录聚合酶链反应(RT-PCR)检测肝组织中SMA、Ⅰ、Ⅲ胶原和转化生长因子β1(TGF-β1)下游信号Smad3 mRNA的表达。结果抗纤二号治疗组B和C与模型组比较,体重较重,肝脏、脾脏变小,肝重/体重和脾重/体重降低(P<0.05)。病理学观察,抗纤二号治疗组B显著逆转了免疫性大鼠的肝纤维化。苏木精-伊红染色和Masson染色显示抗纤二号治疗组B胶原明显减少(P<0.05)。逆转录聚合酶链反应分析SMA、Ⅰ、Ⅲ型胶原和Smad3 mRNA的表达在抗纤二号治疗组均明显减少(P<0.05)。免疫组织化学观察SMA的表达在抗纤二号治疗组均明显降低,同时分析表明Smad3 mRNA与Ⅰ、Ⅲ胶原mRNA存在正相关(r=0.890)。结论抗纤二号能逆转免疫性肝纤维化,这是由于能部分抑制肝星状细胞的增殖和抑制肝纤维化有关的细胞因子TGF-β1下游信号Smad3的表达。
Objective On the basis of the lasted clinical experience, our group will discuss the treatmented mechanism of Chinese herb kang-xian-er-hao （KXEH） ameliorate hepatic cirrhosis. Methods Male wistar rats were divided into five groups, excepted for normal group N, the remnant four groups were all given intraperitoneal injection of porcine serum （0.5 ml/time, 2 times/week, total 12 weeks）. In KXEH early treatment group B, the rats were fed with KXEH by gavage, 1 g/100 g, once a day at the third week. In KXRH late treatment group C, the rats were fed with KXEH by gavage, 1 g/100 g, once a day at the ninth week. In γ interferon treated group D the rats were subcutaneous injection γ-interferon（0.1 million） every day at the ninth week. The model group A and normal group N were fed with the same amount of saline by gavage. The rats were killed at the end of the twelfth weeks, the formation of liver fibrosis was observed with HE stain and Masson stain. The expression of Smooth muscle actin（SMA） was observed by immunohistochemistry. As well as SMA,collagen Ⅰ ,Ⅲ mRNA and Smad3 mRNA, which is TGF-β1 downstream signal, were detected in liver samples with RT-PCR assay. Results In KXEH treated group B and C, the body weight was heavier,the size of liver and spleen was smaller and the ratio of liver weight/body weight and spleen weight/body weight was decreased compared with the model group A（P〈0.05）. Upon pathological examination and with HE stain and Masson stain assay, the KXEH treated groups had some reverse effects on the rat＇s fibrotic liver. In group B the grade of liver fibrosis was decreased apparently compared with the model group A（P〈0.05）. On the rat＇s fibrotic liver, the expression of type-Ⅰ , type-Ⅲ collagen SMA and Smad3 mRNA was markedly reduced in the KXEH treated group B as compared with the model group A（P〈0.05）. Moreover the expression of SMA through the immunohistochemistry assay was obviously decreased in the KXEH treated groups. A high relativity was found b
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2005年第5期321-324,共4页
Chinese Journal of Infectious Diseases
