摘要
碳纳米管是一种纳米尺度的新型碳材料,具有独特的物理、化学性质。近年来,碳纳米管在生物医学领域的潜在应用前景已经引起科学界和产业界的极大兴趣与关注。与蛋白质分子的非特异性结合是碳纳米管应用于生物系统中必须考虑的基本问题之一。本研究应用扫描电镜和酶联免疫法作为评价方法,定性和定量地分析了血浆中重要凝血因子纤维蛋白原在单壁碳纳米管薄膜表面的非特异性吸附行为;同时,采用聚乙二醇(PEG)分子对单壁碳纳米管薄膜(SWNT膜)进行了表面修饰,通过X-光电子能谱(XPS)对材料表面的化学组成进行了表征,并初步探讨了PEG修饰对纤维蛋白原分子在SWNT膜表面非特异性吸附的阻止作用。实验结果表明,纤维蛋白原分子在SWNT膜表面有强烈的非特异性结合,吸附于薄膜表面的纤维蛋白原分子仍然保有自身的免疫原性。SWNT膜表面可以被PEG分子修饰,连接在薄膜表面的PEG分子可以在一定程度上抑制一定浓度范围内的纤维蛋白原分子的非特异性结合。
In recent years, carbon nanotubes have attracted intensive interests of potential applications in biomedical area because of their unique nanostructures and outstanding mechanical,electrical properties. Fibrinogen is one kind of important human plasma proteins related to blood coagulation. This work is focused on the adsorption of fibrinogen to the SWNT nonwoven film fhrough nonspecific binding. The adsorption behavior of fibrinogen was analyzed using scanning electron microscope （SEM） and enzyme linked immunosorbent assay（ELISA） respectively. Experimental results indicated that SWNT nonwoven film exhibited intense adsorption preference to fibrinogen molecules. Data obtained from ELISA demonstrated that the immunogenicity of adsorbed fibrinogen molecules was well maintained. Analytical results from XPS indicated that PEG modification of SWNT nonwoven film was achieved and the nonspecific adsorption of fibrinogen on SWNT nonwoven film was partly inhibited by the PEG modification.
出处
《中国生物医学工程学报》
EI
CAS
CSCD
北大核心
2005年第6期763-766,783,共5页
Chinese Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(3027039490306004)
