摘要
文中采用醋酸锂转化法，将一段目的基因转入到酵母体内，破坏酵母乙醇脱氢酶Ⅱ基因的表达。通过对目的基因的扩增和乙醇脱氢酶Ⅱ酶活检测验证基因敲除情况，并通过传代抗性试验验证突变株遗传稳定性良好。
In this paper, we transformed a gene into yeast genome so as to delete Alcohol Dehydrogenase Ⅱ （ADH Ⅱ ） by LiAc method. The mutant gene could be identified by PCR and test enzyme activity of ADH Ⅱ . The mutant strain inheritance was well by transfer of culture in addition of G418 inhibitor.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2005年第11期30-33,共4页
Food and Fermentation Industries
