摘要
目的:探讨银杏叶提取物(ginkgobilobaextract,GBE)对脑缺血后梗死体积及神经元凋亡相关蛋白Bcl-2,Caspase-3表达的影响。方法:将SD大鼠随机分为缺血对照组、小剂量GBE治疗组(小剂量组)、大剂量GBE治疗组(大剂量组)。采用大鼠大脑中动脉栓塞模型,应用光镜、TTC染色、免疫组织化学染色及TUNEL染色检测方法,在不同时间点观察各组大鼠脑梗死体积的变化以及Bcl-2,Caspase-3阳性细胞表达的不同和神经细胞凋亡的情况。结果:在不同时间点各给药组大鼠的脑梗死体积小于缺血对照组,差异有显著性(P<0.01);大剂量组脑梗死体积与小剂量组脑梗死体积相比差异亦有统计学意义(P<0.05)。各给药组大鼠Bcl-2阳性细胞表达数目均高于缺血对照组,而Caspase-3阳性细胞表达数目则低于缺血对照组(P<0.01);大剂量组阳性细胞表达数目与小剂量组阳性细胞表达数目相比差异亦有显著性(P<0.05)。结论:银杏叶提取物可减少脑梗死体积,在脑缺血损伤急性期可增加Bcl-2的表达而减少Caspase-3的表达。
Objective To explore the protective effects of ginkgo biloba extract（GBE） on local cerebral ischemia reperfusion injury and its influence on the expression of bcl-2 and caspase-3. Methods The model of middle cerebral artery ischemia and reperfusion was set up. 60 SD rats were randomly divided into 3 groups： A （ischemia group）, B （small-dose GBE treatment group） and C （high-dose GBE treatment group）. The brain sections were used for HE, TUNEL staining and bcl-2 and caspase-3 immunohistochemical staining. Meanwhile, the sections were stained by TIC for surveying the infarct volume. Results The infarct volumes and apoptotic cells of group B and C were significantly less than group A （ P 〈 0. 01 ）, those of group C were less than of group B（ P 〈 0. 05）. The expression of bcl-2 positive cells in group B and C were significantly higher than group A （ P 〈 0. 01）, whereas the expression of caspase-3 positive cells in group B and C were significantly less than group A （ P 〈 0. 01 ）. There was significant difference on the expression of bcl-2 and caspase-3 positive cells between group B and C （ P 〈 0.05）. Conclusion GBE could decrease infarct volumes and the number of apoptotic cell after ischemia and reperfusion by up-regulatlng bcl-2 and down-regulating caspase-3 protein.
出处
《实用医学杂志》
CAS
2005年第23期2616-2618,共3页
The Journal of Practical Medicine
