摘要
目的:研究肝纤维化模型大鼠肝组织中Ⅰ、Ⅲ型胶原含量变化情况,探讨不同剂量小柴胡汤对实验性模型大鼠肝纤维化程度的影响。方法:肉眼观察、常规HE染色法和免疫组化染色法观察小柴胡汤对实验性肝纤维化模型大鼠肝组织中Ⅰ、Ⅲ型胶原含量的影响。结果:6、12周肝纤维化模型组Ⅰ、Ⅲ型胶原含量与正常对照组比较显著增加(P<0.01);12周肝纤维化模型组Ⅰ、Ⅲ型胶原含量较6周肝纤维化模型组显著增加(P<0.05)。小柴胡汤治疗组与同期肝纤维化模型组相比,肝纤维化程度显著减轻(P<0.05),Ⅰ、Ⅲ型胶原含量明显减少(P<0.05)。不同剂量小柴胡汤组间Ⅰ、Ⅲ型胶原含量比较无显著性差异(P>0.05)。结论:肝纤维化发生、发展过程中,Ⅰ、Ⅲ型胶原含量显著增加,小柴胡汤能减轻模型大鼠肝纤维化程度,下调Ⅰ、Ⅲ型胶原表达。
Objective： to investigate the contains of collagen Ⅰ and Ⅲ in hepatic tissue of rats hepatic fibrosis. To study the effect of different doses of xiaochaihu decoction on rats hepatic fibrosis. It is expected that the mechanism how to prevent experimental hepatic fibrosis by xiaochaihu decoction can be disclosed through this experiment. Methods： the hepatic fibrosis rats model were induced by introperitoneal injection of interfered with different doses of xiaochaihu decoction. The contains of collagen Ⅰ and Ⅲ was checked by immunalhistochemistry. Results：on the 6th and 12th weeks, the contains of collagen Ⅰ and Ⅲ in model groups increased significantly compared with normal control group,on the 12th week, contain of collagen Ⅰ and Ⅲ in model groups increased significantly compared with the 6th week model group. In comparision with model group of the same time, the degree of proliferation of collagenous fibers in the liver reduced significantly in the therapeutic groups. There was no difference in the collagen Ⅰ and Ⅲ among the three therapeutic groups of the same time. Conclusions：in the course of the hepatic fibrosis, contains of collagen Ⅰ and Ⅲ increases. Xiaochaihu decoction could significantly reduced the proliferation degree of collagenous fiber.
出处
《山东中医药大学学报》
2005年第4期316-318,共3页
Journal of Shandong University of Traditional Chinese Medicine
关键词
肝纤维化
小柴胡汤
Ⅰ型胶原
Ⅲ型胶原
hepatic fibrosis
xiaochaih u decoction
collagen Ⅰ
collagen Ⅲ
