摘要
目的 探讨同型半胱氨酸硫内酯(HcyT)对人脐静脉内皮细胞株(HUVEC)的损伤作用及其可能机制。方法 采用倒置显微镜、四唑盐(MTT)比色法及碘化吡啶(PI)染色流式细胞仪检测HeyT对细胞毒性作用，以荧光标记流式细胞仪检测细胞内反应性氧类(ROS)的生成。结果 一定剂量HcyT刺激后，内皮细胞发生凋亡，且凋亡呈浓度时间依赖性。随着HcyT浓度的增加，ROS的生成逐渐增加。结论 HcyT通过ROS呈时间浓度依赖性地诱导内皮细胞凋亡，这可能是Hey致血管病变的机制之一。
Objective To study the possible effect of reactive homocysteine derivative of homocysteine thiolactone （HcyT） on apoptosis and reactive oxygen species （ROS） production in human umbilical vein endothelial cells （HUVEC）. Methods Cultured vascular endothelial cells were treated with HcyT at various concentrations. Light microscopy and flow cytometry were applied to detect and observe cell injury and production of ROS. Results HcyT induced cell death and cell apoptosis, which occurred in a time- and concentration-dependent manner. HcyT treatment also increased intracellular ROS, which coincided with the increased HcyT concentration. Conclusion HcyT exerted its genotoxic effects on HUVEC through an apoptotic pathway, which might be mediated by increased intracellular ROS.
出处
《中华老年多器官疾病杂志》
2005年第3期200-203,共4页
Chinese Journal of Multiple Organ Diseases in the Elderly
