摘要
对普通小麦抗条锈新种质-体克2号进行了遗传学分析和RAPD标记研究。结果表明,体克2号对条中32号生理小种的的抗性是由1对显性基因控制的。RAPD分析筛选出重复性强、在抗病亲本和抗性基因池稳定出现的特异DNA片段2个,即引物S369的扩增片段和引物S1397的扩增片段,其长度分别约为770bp和1400bp。利用Mapmaker3．0对引物S369扩增出来的特异片段与目的基因的遗传连锁性进行分析,该多态性差异与目的基因的连锁距离为10．4cM。
Wheat new germpasm Tike2 with stripe rust resistance was studies by genetics and RAPD techniques. The result showed that the resistance to physiological race tiaozhong32 is controlled by one dominant gene. 2 additional DNA fragments were amplified repeat and steadily in resistant parent and resistance gene pool by RAPD technique, that is the fragments amplified respectively by the primer S369 and S1397. The additional DNA fragment amplified by the primer S369 was about 770bp, and the fragment by S1397 was about 1400bp. Analyzed the genetic distance of the additional DNA fragment by the primer S369 with the aim gene by the Mapmaker 3.0 software, it is 10.4 cM.
出处
《中国农学通报》
CSCD
2005年第11期123-125,共3页
Chinese Agricultural Science Bulletin
基金
国家973项目"水稻和小麦抗主要真菌病害的分子遗传学基础"(G2000016202)国家863项目"小麦高效分子育种体系的建立及优质
多抗性品种选育"(2003AA207100)陕西省科技攻关项目"小麦抗病材料创新与生物技术育种"(2003K03一G1-04)2002年西北农林科技大学青年基金"小麦抗条锈新种质体克2号抗性基因的RAPD分析"(0808)资助。
