摘要
目的:对卵巢皮质间质细胞增生癌基因和抑癌基因的表达及伴发癌的癌基因和抑癌基因进行免疫组织化学检测探究。方法:对40例卵巢皮质间质细胞增生伴有子宫内膜癌、卵巢癌,平滑肌瘤、内膜息肉等疾病增生的卵巢皮质间质细胞进行免疫组织化学C-erbB-2、Rb、p53、PCNA的检测,并对手术病例进行术后血E2、p检测,同时观察内膜癌C-erbB-2、Rb、p53、PCNA癌基因蛋白、肿瘤细胞增殖的表达。结果:在卵巢皮质增生细胞C-erbB-2阳性率为58%,Rb阳性率为30%,p53阳性率为2.5%,PCNA阳性率为33%,子宫内膜癌细胞C-erbB-2阳性率为100%,Rb阳性率为85%,p53阳性率为80%,PCNA阳性率为85%。结论:伴有子宫内膜癌、卵巢癌的卵巢增生的皮质间质细胞C-erbB-2、Rb表达阳性,在子宫肌瘤等良性病变C-erbB-2和PCNA表达阳性,p53在增生间质细胞很少表达。血E2和P检测正常。而在子宫内膜癌细胞各项免疫组化染色多呈阳性。
Objective：To investigate the expression of cancer gene and anti-oncogene in proliferating stromal cells of ovary cortex in the patients with cell proliferation of ovary cortex and complicated cancers. Methods ：The malignant tumors were classified according to the morphology.Immunohistochemistry staining was used to measure the expression of C-erbB-2, Rb,p53 and PCNA.The blood levels of E2 and P were examined after operation. Results：The immunohistochemistry measurement showed that the positive rate of C-erbB-2,Rb,p53 and PCNA in the proliferating stromal cells of ovary cortex and the tumor cells of endometrium carcinoma was 58%and 100% ,30%and 85% ,2.5%and 80% ,33%and 85% ,respectively. Conclusion： C-erbB-2 and Rb are posive in the proliferating stromal cells in the cases of cell proliferation of ovary cortex complicated with endometrium carcinoma or ovary carcinoma ,while C-erbB-2, Rb, p53 and PCNA are all positive in the tumor cells of endometrium carcinoma.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第12期921-922,958,F0004,共4页
Journal of Nanjing Medical University(Natural Sciences)
关键词
卵巢
间质细胞增生
免疫组织化学
内膜癌
平滑肌瘤
stromal cell proliferation of ovary cortex
immunohistochemistry
endometrial carcinoma
and leiomyoma.
