摘要
目的 探讨四氢生物喋呤(BH_4)对人脐静脉内皮细胞产生一氧化氮(NO)和超氧阴离子(O_2^(?))的影响。方法 在培养液中分别加入不同浓度的D—葡萄糖、胰岛素和BH_4，24h后取细胞培养液分别测定一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)活性、NO和O_2^(?)浓度。结果 BH_4(10、100、500μmol／L)使内皮细胞NOS活性增高，500μmol／L BH_4使内皮细胞NO产生增加，10或100μmol／L BH_4对内皮细胞产生NO有增加的趋势，但与对照组比较无显著性差异(P＞0.05)；25mmol／L葡萄糖+BH_4(10、100、500μmol／L)对内皮细胞产生NO与对照组比较无显著性差异(P＞0.05)；高浓度胰岛素(10、100、1000mU／L)+BH_4(10、100、500μmol／L)使内皮细胞NOS活性增强，NO产生增加。BH_4(10、100、500μmol／L)对内皮细胞SOD活性无明显影响，但可以改善25mmol／L葡萄糖对内皮细胞SOD活性的影响；胰岛素+BH_4对内皮细胞SOD活性无明显影响(P＞0.05)。BH_4(10、100、500μmol／L)使内皮细胞产生O_2^(?)减少，并可以改善25mmol／L葡萄糖对内皮细胞产生O_2^(?)影响；胰岛素+BH_4组O_2^(?)浓度明显低于对照组和不同浓度胰岛素组(P＜0.01)。结论 BH_4可以增加培养的人脐静脉内皮细胞NOS活性，使NO产生增加而使O_2^(?)水平下降。
Objective To investigate the effects of tetrahydrobiopterin（BH4 ） on release of nitric oxide（NO） and superoxide anion（O2 ） in human umbilical vein endothelial cells（HUVECs）. Methods HUVECs were incubated with different concentration of D-glucose, insulin and BH4 , NOS, SOD activity, and the concentration of NO and O2 were measured in the supernatant. Results As compared with controls, NOS activity, the concentration of NO and O2 were elevated, while SOD activity was decreased in the HUVECs culture supernatant in the presence of 25 mmol/L D-glucose. In the presence of 10,100,1 000 mU/L insulin, NOS activity, the concentration of NO was increased, with little changes in SOD activity and O2. BH4 attenuated NOS activity, concentration of NO and decreased O2 with no change in SOD activity. Furthermore, BH4 reverse of the elevated O2 induced by glucose. Conclusion BH4 may in crease NOS activity, induce NO generation and decreased the generation of O2.
出处
《高血压杂志》
CSCD
北大核心
2005年第11期706-710,共5页
Chinese Journal of Hypertension
