摘要
以P123嵌段共聚物表面活性剂为模板剂制备介孔氧化硅SBA-15,并用沉积-沉淀(DP)法在SBA-15介孔表面负载纳米Au颗粒制备得到金复合介孔SBA-15材料(Au-SBA-15).再以Au-SBA-15材料制备玻碳修饰电极,将血红蛋白固定于修饰电极上用循环伏安法考察其对不同浓度H_2O_2溶液的电催化反应.在固定了血红蛋白的Hb/Au-SBA-15/GC修饰电极上,H_2O_2在+0.95V处出现了氧化峰,且随着H_2O_2浓度的增大峰电流不断增加,说明金复合介孔氧化硅材料具有良好的生物兼容性,有利于血红蛋白的固定,其修饰电极对H_2O_2溶液具有一定的电催化作用.
Mesoporous SBA-15 was prepared by using Pluronic P123 triblock copolymer as structure-directing agents and Au-modified mesoporous SBA-15 （Au-SBA-15） was synthesized by a Deposi- tion-Precipitation methods. The surface of glass carbon was modified with Au-SBA-15 material （named Au-SBA-15/GC electrode）, then hemoglobin （Hb） was immobilized on Au-SBA-15/GC electrode （named Hb/Au- SBA-15/GC electrode）. The Hb/Au-SBA-15/GC electrode was applied to electrochemical determination of different concentration of H202 solution. An oxidation peak at 0.95 V was observed, and the peak current was found to be increased with H2O2 concentration increasing. The results indicate that the Hb/Au-SBA-15/GC modified electrode exhibited certain electrocatalytic activity.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2005年第23期2117-2120,共4页
Acta Chimica Sinica
基金
国家自然科学基金(Nos.20236010
20476025)
上海市教委资助项目.
