摘要
采用毛细管电泳淌度移动法测定了β-环糊精与芦丁和氯霉素这两种药物的包合常数及包合反应的热力学参数。所用未涂层熔融石英毛细管的规格为内径50μm,总长37cm,有效长度30cm,电泳缓冲液为25mm o l/L硼砂+100mm o l/L硼酸(pH8.6),并加入不同浓度的-βCD。在25℃时测得芦丁和氯霉素与β-CD的包合常数分别为98.5和1 204L/m o l。通过测定不同温度下的包合常数,求得包合反应的系列热力学参数。结果表明-βCD与两种药物的包合反应焓熵互补现象明显。采用MM2力场对-βCD与两种药物分子的包合物的稳定构型进行了分子力学模拟,模拟结果与实验结果相符。
The interactions of two medicines of rutin and chloramphenicol with β-cyclodextrin were investigated using mobility shift method by capillary electrophoresis. The size of the capillary column was 37cm×50μm i.d. with an effective length of 30 cm. The running buffer was a mixture of 25mmol/L sodium borate and 100mmol/L boric acid （pH 8.6）. Effective mobilities of the two medicines changed with altering the concentration of β-cyclodextrin. By nonlinear least square fitting curve, the inclusion constants of 98.5 and 1204L/mol for rutin and chloramphenicol were obtained at 25℃, respectively. The inclusion constants at different temperatures were determined, from which a series of thermodynamic parameters were derived. Experimental results show that an obvious relationship of mutual compensation between enthalpy and entropy existed in these inclusion reactions. The most stable configurations of the inclusions were calculated using MM2 force field, and the results were well in agreement with the experimental results.
出处
《色谱》
CAS
CSCD
北大核心
2005年第6期673-676,共4页
Chinese Journal of Chromatography
基金
山东省教育厅科技计划项目(03A06)
关键词
毛细管电泳
Β-环糊精
芦丁
氯霉素
包合常数
热力学参数
capillary electrophoresis
β-cyclodextrin
rutin
chloramphenicol
inclusion constants
thermodynamic parameters
