摘要
目的观察大鼠血肿周围脑组织IL-1β蛋白表达的变化规律,并探讨抑肽酶的脑保护作用。方法尾状核注射胶原酶诱导大鼠脑出血(ICH)模型,分别用免疫组化法和ELISA法测定IL-1β的蛋白表达水平,参照Berderson's法进行神经功能缺损评分。结果模型组血肿周围脑组织的IL-1β阳性细胞数和蛋白含量在6 h即有增加,48 h左右达高峰,而抑肽酶组相应各时间点的阳性表达明显减少,尤以48、72 h较为明显;模型组与抑肽酶组在61、2 h的神经功能缺损评分无显著性差异,其余各时间点均有显著性差异(P<0.01)。结论IL-1β参与了脑出血后的继发性脑损伤,抑肽酶能够明显减轻脑出血后的IL-1β表达,从而发挥脑保护作用。
Objective To investigate the effect of aprotinin on IL-1β expression of perihematoma tissue （PHT） after intracerebral hemorrhage in rats and explore the protective mechanism of aprotinin. Methods Intracerebral hemorrhagic model was induced to utilize the local injection of collagenase into the basal ganglia with sterile. In different times, the interleukin-1β protein expression was detected by immunohistochemistry and ELISA and neurological severity scores was measured with Berderson＇s method. Results In model group （MG）, the IL-1β protein content and positive cells in PHT began to increase slightly at 6 hours compared with sham group, reached the peak at 48 hours. In aprotinin-treated group（ATG）, aprotinin reduced the positive cells and content,especially at 48 hours,72 hours, and 7 days （compared with MG, P〈0. 01）. It showed no significant difference was between MG and ATG at 6 hours and 12 hours, however and there was greatly difference at 24,48,72 and 168 hours between these two groups （P〈0. 01）. Conclusions IL-1β attaches itself to the secondary damage after ICH and aprotinin could reduce the IL-1β expression in making protective effect on ICH.
出处
《卒中与神经疾病》
2005年第5期288-290,共3页
Stroke and Nervous Diseases
关键词
脑出血IL-1β抑肽酶
Intracerebral hemorrhage Interleukin-1β Aprotinin
