摘要
对油菜叶片及其外植体所产生的愈伤组织和分生组织细胞进行了详细的透射电镜观察。观察表明,它们的超微结构发生了深刻变化,而且这些变化与细胞脱分化和再分化有关。它们的主要区别是:油菜叶肉细胞是一种典型的薄壁细胞,细胞质很少,但叶绿体非常丰富。脱分化的愈伤组织细胞类似于薄壁细胞,中央也有一个大液泡,液泡周围有一层含有一些细胞器的细胞质。质体已无片层结构和嗜锇小球,但常有大量的贮藏淀粉。分生细胞与愈伤组织细胞相比,体积较小,细胞质较浓,线粒体、高尔基体、核糖体、内质网和质体非常丰富。它没有中央液泡,只有许多小液泡,而且小液泡中常有一种电子密度很高的物质,这种物质随培养时间的加长而增多。质体无淀粉粒和片层结构,但基质中的管状和泡状物质及嗜锇小球显著增多。
Cell ultrastructures in rape(Brassica napus L.)leaves and their calli,andmeristemoids were studied by transmission electron microscopy in detial.Our resultsshowed that their ultrastructures had many profound changes,the main differences ofthese changes were that the rape mesophyll cell was a kind of typical parenchyma cellwith many chloroplasts and a large central vacuole around which there was a littlecytoplasm.The callus cell derived from the rape mesophyll cell resembled parenchymacell of intact plant and there was also a central vacuole and a layer of cytoplasm withsome organelles.The lamellar system and osmiophilic globuli had disappeared,butthere was abundon stored starch in the plastid.The meristematic cell was small in size,more densely cytoplasmic and richer in mitochondria,Golgi bodies,ribesomes,endoplasmic reticulum and plastids compared with the callus cell.These cells did notpossess central vacuoles.However,they had many small vacuoles in which there wasa kind of electron-dense material whose amount increased with time in culture.Theymay be phenolic.The stored starch and lamellar system were usually absent but the vesicle-shaped and tube-shaped elements and osmiophilic globuli obviously increasedin the plastid.These changes of cell ultrastructure had a close relation to the cellde-differentiation and re-differentiation during the plantlet regeneration from leaf seg-ment of rape.
出处
《作物学报》
CAS
CSCD
北大核心
1989年第2期146-150,共5页
Acta Agronomica Sinica
关键词
油菜
叶肉细胞
脱分化
再分化
Rape mesophyll cell
Callus cell
Meristematic cell
De-differentiation Re-differentiation
Ultrastructural change
