摘要
目的:了解黄芪注射液对体外培养的人腹膜间皮细胞转化生长因子-β1(TGF-β1)和碱性成纤维细胞生长因子(bFGF)分泌及其mRNA表达的影响。方法:取择期手术患者的网膜间皮细胞进行体外培养传代,第三代细胞用于实验,待细胞同步后分为五组(对照组、腹膜透析组、黄芪1组、2组和3组)观察。单四唑(MTT)法检测各组间皮细胞的增殖活性。用ELISA法检测细胞上清液中TGF-β1和bFGF的含量,并进行比较。采用逆转录多聚酶链式反应(RT-PCR)法检测两种细胞因子mRNA表达情况并进行比较。结果:腹膜间皮细胞在PDS干预下还原能力明显下降,A值显著低于对照组和黄芪组(P<0.05);分泌TGF-β1和bFGF明显增加,与对照组比较,P<0.05。腹膜间皮细胞经含不同浓度黄芪注射液的PDS干预后,TGF-β1和bFGF分泌与PDS组比较显著下降(P<0.05),黄芪2组和3组显著低于黄芪1组(P<0.05),黄芪3组和2组间比较无显著差异(P>0.05)。腹膜间皮细胞在PDS干预下TGF-β1和bFGF mRNA表达明显升高,分别比对照组上升62.43%和37.68%。黄芪组TGF-β1和bFGF mRNA表达显著低于PDS组(P<0.05);黄芪2组和3组表达显著低于黄芪1组(P<0.05),黄芪3组和2组间比较无显著差异。结论:黄芪注射液具有抑制PDS导致的腹膜间皮细胞TGF-β1和bFGF过度分泌和表达的作用。
Objective： To investigate the effect of Astragalus mongholicus injection （AM） on the secretion and mRNA expression of transforming growth factor （TGF-β1） and basic fibroblast growth factor （bFGF） in cultured human peritoneal mesothelial cells （HPMC）. Methods： HPMC got from patients underwent surgical operation were cultured in vitro. At first, cells from the third passage were incubated with RPMI1640 culture medium containing 0.1% FBS for 24 hours. Then, they were divided into control group, PDS group, AM group 1, AM group 2 and AM group 3 for testing. Each group was supplemented with equal volum of RPMI1640 culture medium containing 20% FBS. After 24 hour incubating, mitochondrial dehydrogenases activity （MTT assay）, the levels of TGF-β1 and bFGF in supernatant of the cell culture and the mRNA expression of TGF-β1 and bFGF were detected. Results： Significant decrease of mitochondrial dehydrogenase activities were observed in PDS group as compared with those in control group and AM groups （ P 〈 0.05 ）. No significant difference of mitochondrial dehydrogenases activity were found among AM groups （P 〉0.05 ）. The levels of TGF-β1 and bFGF in supernatant of the cell culture were significantly lower in control group than those in PDS group. Marked lower levels of TGF-β1 and bFGF were found in AM group 1, AM group 2 and AM group 3 as compared with those in PDS group （ P 〈 0.05 ）. The level of TGF-β1 and bFGF in AM group 1 were significantly higher than those in AM group 2 and AM group 3 （P 〈0.05 ）. The levels of TGF-β1 and bFGF in AM group 2 were higher than those in AM group 3, but no significant differences were found between them （ P 〉 0.05 ）. The mRNA expressions of TGF-β1 and bFGF in PDS in control group. And significant mRNA expressions compared with those in AM groups （ P 〈 0.05 ） TGF-β1 and bFGF induced by commercial PDS group increased significantly as compared with those of TGF-β1 and bFGF were Conclusion ： The secretion found in PDS gr
出处
《医学研究生学报》
CAS
2005年第11期972-976,共5页
Journal of Medical Postgraduates
基金
江苏省社会科技发展基金资助项目(批准号:BS2002003)
关键词
黄芪
腹膜间皮细胞
转移生长因子-Β1
碱性成纤维细胞生长因子
Astragalus mongholicus
Human peritoneal mesothelial cell
Tranforming growth factor-β1
Basic fibroblast growth factor
