摘要
利用ＤＮＡ解旋荧光测定法（ＦＡＤＵ）研究了低剂量γ射线诱导的淋巴细胞ＤＮＡ断裂的适应性反应，并观察了不同浓度的３－氨基苯甲酰胺（３－ＡＢ）对适应性反应的影响。结果表明：０．５～４．０ｃＧｙγ射线照射（Ｄ１），均可诱导淋巴细胞对１５Ｇｙγ射线照射的抗性，８．０ｃＧｙ引起的适应性反应不明显，２．０，４．０ｃＧｙ的γ射线为诱导适应性反应的最适剂量。５～２０Ｇｙ的Ｄ２照射均可显现出Ｄ１（２．０ｃＧｙ）诱导的适应性反应，以１５Ｇｙ照射后适应性反应最强。０．５ｍｍｏｌ／Ｌ的３－ＡＢ就可明显地抑制适应性反应。当浓度增至５．０ｍｍｏｌ／Ｌ时，适应性反应几乎完全受抑。
Fluorometric analysis of DNA unwinding was used to study the adaptive response of DNA strand breaks induced by low dose γ-rays and the effect of pADPRT inhibitor-3-AB on the adaptive response. The results indicated that 0. 5-4 cGy γ-rays could induce adaptive response of DNA strand breaks in lymphocytes , especially at the doses of 2.0 and 4. 0 cGy. This response was not obvious after 8. 0 cGy γ-rays irradiation. A challenge dose of 5-20 Gy could make the response expressed , 15 Gy was the best one and 30 Gy was too high to give an adaptive response. 0. 5 mM 3-AB could inhibit the response vigorously. As the concentration increased, the adaptive reaponse could be inhibited completely.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
1996年第1期32-34,共3页
Chinese Journal of Radiological Medicine and Protection
