摘要
目的观察骨膜细胞联合BMP协同成骨作用及其效果,探索骨缺损后骨再生的新方法。方法采用自体骨膜细胞体外培养、扩增、传代,观察兔骨膜细胞活细胞形态学和功能变化;兔24只随机分四组,切除双侧连同骨膜桡骨1 cm,分别置入骨膜细胞+BMP、骨膜细胞、BMP、空白对照,术后定期进行X线拍片、组织学检查。结果兔骨膜培养活细胞形态分三期:细胞分裂期、细胞发展期、细胞功能期,细胞传代进化后,其结构与原代细胞无明显差异;骨膜细胞体外培养,大量增殖,其细胞悬液自体移植至骨缺损区能形成骨组织;修复骨缺损能力骨膜细胞+BMP>BMP>骨膜细胞,骨膜细胞移植后表现为在骨缺损端形成骨样组织,类似膜内化骨形成新骨,BMP类似软骨内化骨形成新骨,骨膜细胞+BMP表现膜内化骨和软骨内化骨两种机制,以前者明显。结论骨膜细胞或BMP两者均有修复骨缺损的能力,骨膜细胞联合BMP有协同成骨作用,为修复骨缺损的一种理想方法。
Objective To investigate the properties of cultured periosteal cells associated with bone morphogenetic protein （BMP） to find a new treatment for bone defects. Methods The periosteal cells were cultured, expanded and passage cultured in vitro to investigate the characteristics and functional changes of rabbit periosteal cells. Animal models of 1.0cm gaps with complete removal of the bilateral radius medium bone and periosteum were induced in 24 New Zealand rabbits, which were randomly divided into 4 groups to receive the defect repair with periosteal cells combined with BMP, periosteal cells, BMP and blank control respectively. X-ray and histological examination were made periodically after operation to evaluate the effectiveness of bone formation. Results Periosteal cell morphology can be divided into three stages： cell division phase, cell growth phase and cell functional phase. Cellular configuration had no significant difference with primary cells after anagenesis. The callus can be formed after the periosteal cells were transplanted into the bone defection. The osteogenesis ability of periosteal cells combined with BMP was better than that of the BMP, and the osteogenesis ability of the BMP was better than that of the periosteal cells. The mechanism of bone regeneration for periosteal cells was similar to intramembranous ossification, and that of BMP was similar to endochondral ossification. In the periosteal cells combined with BMP group, both intramembranous and endochondral ossification existed, and the former was obvious. Conclusion Both periosteal cells and BMP can improve the repair of bone defect in vivo. Cultured periosteal cells combined with BMP had substantial ability of synergistic osteogenesis, and it was an ideal treatment for bone defection.
出处
《中国医师杂志》
CAS
2005年第10期1345-1348,共4页
Journal of Chinese Physician
