摘要
目的：探讨妊高征患者补体活化与血管内皮细胞损伤之间的关系。方法：采用双抗体夹心酶联免疫吸附法（ＥＬＩＳＡ），检测３５例妊高征患者（妊高征组）和２０例正常孕妇（正常妊娠组）外周血和胎盘后血中补体Ｃ１ｑ、Ｂ因子（ＢＦ）和Ｃ１抑制物（Ｃ１－ＩＮＨ）含量的变化。以Ｐｅｒｃｏｌ密度梯度法分离并计数两组外周血循环内皮细胞（ＣＥＣ）的数量，并对ＣＥＣ与血中Ｃ１ｑ的变化进行了相关分析。结果：（１）妊高征时，血中Ｃ１ｑ含量下降，而ＢＦ和Ｃ１－ＩＮＨ水平升高。这种变化在胎盘后血较外周血更加明显；（２）妊高征时，ＣＥＣ数量明显增加；（３）妊高征时，ＣＥＣ水平与血中Ｃ１ｑ含量呈负相关。结论：补体活化的经典途径和替代途径以及补体调节蛋白都参与了妊高征的发生和发展；补体活化与妊高征血管内皮细胞损伤有关。
Objective:Toevaluatetherelationshipbetweencomplementactivationandvascularendothelialdamageinpregnancyinducedhypertension(PIH).Methods:WequantifiedC1q,B-factor(BF)andC1-inhibitor(C1-INH)inperipheralandretroplacentalseraof35nuliparousPIHpatientsand20normotensivecontrolswithdoubleantibodysandwichELISA.Circulatingendothelialcel(CEC)ofthetwogroupswereisolatedwiththedensitygradientsystemofPercolliquidandcountedundermicroscope.ThecorrelationbetweenCECandC1qwasanalysed.Results:(1)C1qconcentra-tionsinperipheralandretroplacentalseraweresignificantlydecreasedinPIHpatientsthanthatinnormotensivecontrols,however,theconcentrationsofBFandC1-INHweresignificantlyelevatedinPIHpatients.Thechangesinretroplacentalserumweremuchmoreprofoundthanthoseinperipheralserum.(2)ThecountofCECwassignificantlyincreasedinPIHpatientsthanthatincontrols.(3)ThecountofCECwasnegativelycorrelatedtoC1qconcentration.Conclusions:Notonlytheclasi-calcomplementactivationpathwaybutalsothealternativecomplementactivationpathwayandcom-plementregulatoryproteinplayimportantrolesinthepathogenesisofPIH,andcomplementactiva-tionandvascularendothelialdamageiscorelated.EvaluationofC1q,BF,C1-INHandCECmaybeofvalueinclinicalmonitoringandmanagementofPIH.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1996年第3期152-155,共4页
Chinese Journal of Obstetrics and Gynecology
关键词
妊娠高血压
综合征
补体激活
内皮细胞损伤
Pregnancycomplications,cardiovascularHypertensionComplementactiva-tionEndothelium,vascular
