摘要
目的研究孕激素对人子宫内膜腺上皮HOXA11基因表达的影响,初步探讨其影响机制。方法6例增殖期子宫内膜腺上皮细胞进行原代培养,在细胞生长汇合时,分别加入孕酮或孕酮+17β-雌二醇;米非司酮(RU486)预处理后加入孕酮或孕酮+17β-雌二醇培养4、68、d,提取细胞总RNA。用半定量逆转录聚合酶链反应(RT-PCR)方法检测HOXA11基因表达量。结果在原代培养的人子宫内膜腺上皮细胞中加入孕酮或孕酮+17β-雌二醇,第4天时HOXA11基因表达量增加,第6天达高峰,第8天时有所下降;孕酮+雌激素的作用无叠加效应;经RU 486预处理后,上述作用消失。结论孕酮对原代培养的人子宫内膜腺上皮细胞HOXA11基因的表达具有正调控作用,这种作用由孕酮受体介导。
Objective： To examine the effect of progesterone and mifepristone （RU486, which is a progesterone antagonist） on HOXA11 gene expression in human endometrial epithelial cells. Methods： Endometrial samples were obtained from six normal cycling women in the proliferative phase. Endometrial glandular epithelial cells were cultured and treated with progesterone or/and 17βestradiol, or pre-treated with RU486 for two hours, then incubated with progesterone or/and 17β-estradiol for 4, 6, 8 days. Semi-quantitative RT-PCR was used to examine HOXA11 messenger RNA levels. Results： HOXA11 expression was increased in endometrial epithelial cells after treatment with progesterone from the 4th day, peaked at the 6th day, but was equivalent to that of the 4th day at the 8th day. With the pre-treatment of RU486, these changes in HOXA11 expression disappeared. Conclusion： HOXAll gene expression in human endometrial epithelium is up-regulated by progesterone in vitro, and this effect is mediated by progesterone receptor.
出处
《生殖医学杂志》
CAS
2005年第5期292-296,共5页
Journal of Reproductive Medicine
基金
国家自然科学基金资助项目(30471814)
关键词
孕酮
米非司酮
HOXA11基因
子宫内膜腺上皮
半定量逆转录聚合酶链反应
Progesterone, Mifepristone, HOXAll gene
Endometrial epithelium
Semi-quantitative reverse transcriptase-poly merase chain reaction
