摘要
目的观察三七总皂甙(PNS)对大鼠肝脏缺血再灌注损伤线粒体的自由基的清除作用。方法通过阻断血运前将PNS经直接通道注入并保留于肝脏组织的方法并提取肝脏局部缺血再灌注模型大鼠肝细胞线粒体,测定线粒体成分的SOD总活力、MDA含量;且同步观察电镜下的组织学改变。结果PNS组的SOD总活力在再灌注90min时(10.767±1.638)NU/mg·pro明显低于相对应的实验对照组(15.945±2.891)NU/mg·pro(P<0.05)。MDA含量在再灌注90min时(1.822±0.240)nM/mg·pro明显低于相对应的实验对照组(2.893±0.782)nM/mg·pro)(P<0.05)。电镜观察表明PNS组在再灌注90min时线粒体等超微结构的破坏明显较对照组轻。结论PNS在大鼠肝脏缺血再灌注早期主要通过直接清除自由基及提高线粒体SOD活力而减轻和预防大鼠缺血再灌注肝细胞线粒体的损伤。
[Objective] To observe the elimination of oxygen free radicals in rat liver mitochondria by total saponins of panax notoginseng during isehemia reperfusion. [Methods]To infuse and reserve PNS to rat liver though direct channel before blood blocked and then to gain rat mitochondria with isehemia reperfusion. Determining the activity of SOD, MDA and the content of calcium, determining histologie changes by electron microscope. [Results] After 90-minute reperfusion, the activity of SOD in the PNS group （10.767±1.638） NU/mg.pro is significantly lower than that in control group （15.945±2.891） NU/mg.pro （P 〈0.05） and the content of MDA （1.822±0.240） nM/mg.pro is also lower than that in control group （2.893±0.782） nM/mg.pro （P 〈0.05）. More mitochondria injury was observed in PNS group than that in control group. [Conclusion] PNS can protect rat hepatocyte from isehemia reperfusion injury by direct eliminating oxygen free radicals and boosting the activity of SOD, especially during early stage of isehemia reperfusion.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第20期3076-3078,共3页
China Journal of Modern Medicine
