摘要
目的:观察补肾中药对成骨细胞的作用,并探讨最佳的血清制备时间点和含药浓度。方法:①实验于2004-10/2004-12在南方医科大学中医系实验室完成。选用雄性SPF级SD大鼠12只。将大鼠分为4组:高、中、低剂量中药组和空白对照组,每组3只。②高、中、低剂量中药组分别灌胃剂量为每毫升中药中含生药为7.08,3.54,1.77g的补肾中药,2次/d,共3d;空白对照组灌服生理盐水,2次/d,共3d,于末次喂药后45,60及75min采集血清制备含药血清。③用含不同浓度中药血清体外培养成骨细胞,于培养后72h收集培养上清,采用麦芽凝集素沉淀法和放射免疫法测定成骨细胞培养上清骨性碱性磷酸酶(碱性磷酸酶)和骨钙素(骨钙素)含量。④多组间的计量资料比较采用随机分组的方差分析,组间比较采用SNK检验。结果:末次给药后60和75min高、中、低剂量中药组培养上清骨钙素和碱性磷酸酶含量明显高于末次给药后45min(P<0.05),以末次给药后60min时作用最明显。末次给药后45,60,75min中高剂量中药组培养上清骨钙素和碱性磷酸酶含量明显高于相应时间点低剂中药组和空白对照组(P<0.05～0.01)。结论:①补肾中药能够显著促进成骨细胞合成碱性磷酸酶和骨钙素。②在中等浓度组末次给药后60min,补肾中药药效作用最佳。
AIM： To investigate the effects of tonifying kidney Chinese medicine on osteoblast and explore the optimal time point and concentration of containing Chinese medicine of serum prepared. METHODS： ① The experiment was conducted at the laboratory of traditional Chinese medicine, Southern Medical University from October 2004 to December 2004. Twelve SPF .grade male SD rats were ~lected and divided into 4 groups： high, middle and low dosage traditional Chinese medicine group and empty control group with 3 rats in the each group. ② The rats in the high, middle and low dosage traditional Chinese medicine group were treated with tonifying kidney berhs of 7.08,3.54, and 1.77 g raw drug per milliliter hy gastric perfusion, respectively, twice a day for 3 days. Those in the empty control group were treated with gastric peffusion with s＇,dine, twice a day for 3 days. The serum was made at 45, 60 and 75 minutes after last time of taking drugs to prepare containing drug serum. ③ The osteoblast was cultured in vitro with different concentration of Chinese medicine serum, and the cultured supernatant was collected at the 72 hours after culture. The content of supernatant bone alkaline phosphatase （alkaline phosphatase） of osteoblast and osteocalcin （osteocalein） were measured with malt agglutinator sedimentation and radio immunity method. ④ The measurement data in multiple ized analysis of variance, and the formed with SNK test. groups were compared with randomcomparison among groups were per-RESULTS： At 60 and 75 minutes after the last administration, the contents of cultured supernatant osteocalcin and alkaline phosphatase in thehigh, middle and low dosage traditional Chinese medicine group were higher significantly than those at the 45 minutes （P 〈 0.05）, and at the 60 minutes the effect was the most obvious. The contents of cultured supernatant osteocalcin and alkaline phosphatase in the middle and high dosage traditional Chinese medicine group at the 45, 60 and 75 minutes after the last admin
出处
《中国临床康复》
CSCD
北大核心
2005年第34期124-126,共3页
Chinese Journal of Clinical Rehabilitation
