摘要
目的:观察重组人骨形态发生蛋白-2与卵磷脂复合材料修复兔长骨节段性骨缺损的能力,检验重组人骨形态发生蛋白-2的诱导成骨活性及卵磷脂作为重组人骨形态发生蛋白-2载体材料的可行性。方法:实验于2003-12/2004-02在解放军第四军医大学西京医院骨科研究所完成。实验动物由第四军医大学动物研究中心提供,材料由华东基因技术研究所提供。设实验组和对照组两组,24只大耳白兔随机分为两组,每组12只。手术造成桡骨中上段15mm骨缺损,实验组植入重组人骨形态发生蛋白-2与卵磷脂复合材料片,其中重组人骨形态发生蛋白-2的含量为1.0mg,对照组植入单纯卵磷脂片。术后通过影像学、组织学观察及骨密度测定,评价重组人骨形态发生蛋白-2与卵磷脂复合材料对兔长骨节段性骨缺损的修复效果。结果:各组12只兔均纳入最后分析。①骨缺损修复的影像学表现:实验组术后4周时缺损区有大量新生骨痂形成,术后8周时新生骨痂将缺损区桥接,术后12周时达到皮质骨连接;对照组无骨痂形成。②骨缺损修复的组织学表现:实验组术后4周时的骨痂为编织骨,术后8周时骨痂外层为板层骨,中央为编织骨,内有骨髓组织,术后12周时骨痂外层为皮质骨,中央为髓腔组织;对照组缺损区为结缔组织和肌组织充填。③骨密度值比较:实验组术后12周时骨痂密度达到正常值的76%。结论:重组人骨形态发生蛋白-2与卵磷脂复合材料对兔桡骨节段骨缺损具有很好的修复效果,重组人骨形态发生蛋白-2具有良好的成骨活性,卵磷脂可以作为重组人骨形态发生蛋白-2的一种新的载体材料。
AIM： To observe the ability of the composite of recombinant human bone morphogenetic protein-2 （rhBMP-2） and lecithin in repairing the segmental defect of rabbit＇s long bone, and examine the activity of rhBMP-2 in of bone induction and the feasibility of lecithin as a carrier of rhBMP-2. METHODS： The research was done in Orthopedic Institute of Xijing Hospital, the Fourth Military Medical University of Chinese PLA from December 2003 to December 2004. The experimental rabbits were provided by the animal research center of the Fourth Military Medical University of Chinese PLA, and the implanted materials were provided by the Huadong Gene Technology Institute. Twenty-four rabbits were randomly divided into experiment group （n=12） and control group （n=12）. A 15-mm defect was made in middle-superior segment of rabbits＇ radius, and then in experiment group the composite of rhBMP-2 and lecithin was implanted into the defect, in which the content of rhBMP-2 was 1.0 mg, and in the control group only lecithin was implanted. The effect of the composite of rhBMP-2 and lecithin in repairing the segmental defect of rabbit＇s long bone was evaluated by radiograph, histology and bone mineral density detection. RESULTS： All the rabbits with 12 in each group were involved in the analysis of results. ①Radiographic manifestations in the repair of bone defects： In the experimental group, plenty of new callus could be seen in the defected sites at 4 weeks of postoperation; all defect sites were bridged by callus at 8 weeks and connected by cortical bone at 12 weeks; no callus was observed in control group. ② Histological manifestations in the repair of bone defects： In the experimental group, the new callus formed at 4 weeks was woven bone; at 8 weeks, the defected sites were bridged with lamellar bony callus, in witch there was myeloid tissue; at 12 weeks, in the outside of callus the compact bone was formed, and in the center the medullary cavity was seen. In the control group, the defected sites were
出处
《中国临床康复》
CSCD
北大核心
2005年第34期50-52,i0003,共4页
Chinese Journal of Clinical Rehabilitation
