摘要
目的观察慢性心肌损伤过程中小鼠细胞免疫功能的改变。方法BALB/C小鼠随机分成4组,病毒(柯萨奇3m,CVB3m)组、毒素(T鄄2)+病毒组、毒素组和对照组。观察各组小鼠脾淋巴细胞亚群及其在刀豆蛋白A(CoA)刺激下增殖及白细胞介素鄄2(IL鄄2)的分泌水平。结果毒素+病毒组小鼠心肌形成慢性损伤,感染病毒7 d时,毒素+病毒组脾淋巴细胞亚群、淋巴细胞增殖反应及IL鄄2的分泌水平均低于病毒组,14 d后虽有不同程度的提高,但低于病毒组最高值。结论T鄄2毒素染毒小鼠感染CVB3m后,心肌出现慢性损伤,这可能是由于淋巴细胞数量、功能和活性异常,细胞免疫功能紊乱,导致发病高峰延迟,阻碍早期清除病毒及杀伤受染细胞的作用有直接关系。
Objective To investigate the change of cellular immune functions during chronic myocardial damage in mice. Methods Mice were randomly divided into 4 groups： viral group I ,toxin ＋ virus group Ⅱ , toxin group Ⅲ and control group Ⅳ. The lmyphocyte subset, IL-2 content and lymphocyte proliferation were observed. Results The chronic myocardial damage was found in group Ⅱ . Seven days after virus infection, the lymphocyte subpopulation,IL-2 content and lymphocyte proliferation ability responsive to CoA stimulation were significantly lower than those in group Ⅲ. After 14 days, the level of above-mentioned three indexes increased in different degrees, but still showed inadequate content compared with the highest level in group I . Conclusions The chronic myocardial damage induced by CVB3m in T-2 infected mice might be related to the down-regulation of lymphocyte number, function, activity and abnormal cellular immune function and delay the onset of disease and encumber the early elimination to virus and infected cells.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2004年第1期36-38,共3页
Chinese Jouranl of Endemiology
基金
黑龙江省卫生厅资助项目(99116)
