摘要
目的检测不同病理类型狼疮性肾炎(lupusnephritis,LN)患儿肾组织细胞凋亡与增殖,分析LN患儿肾组织细胞凋亡与增殖的关系。方法用原位末端标记法(TUNEL)检测21例Ⅳ型、6例Ⅴ型LN患儿肾组织及9例对照肾组织中的细胞凋亡,用免疫组织化学法检测其细胞增殖(PCNA,增殖细胞核抗原),同时用免疫组织化学及图像分析法检测细胞凋亡相关基因蛋白PDCD5、Caspase-3的表达。结果1.Ⅳ型LN患儿肾小球凋亡细胞数(0.93±0.28)、增殖细胞数(9.97±1.90)及P/A值(11.15±2.49)均明显高于Ⅴ型LN患儿相应指标(0.66±0.11),(4.82±1.46)和(7.25±1.71),P<0.05,P<0.01和P<0.01。2.Ⅳ型和Ⅴ型LN患儿肾组织PDCD5、Caspase-3表达:PDCD5在Ⅳ型LN患儿肾小球的表达强度(0.08±0.02)与V型LN患儿肾小球的表达强度(0.07±0.03)无差异,P均>0.05;Caspase-3在Ⅳ型LN患儿肾小球的表达强度(0.25±0.05)明显高于Ⅴ型LN患儿肾小球的表达强度(0.16±0.03),P<0.01。结论Ⅳ型LN患儿相对于细胞增殖的程度而上调的细胞凋亡远不如Ⅴ型LN患儿;Caspase-3参与LN患儿肾小球细胞凋亡,而PDCD5未起主要作用或其促凋亡作用被抑制。
Objective To detect the apoptosis and the intensity of proliferation in different types of renal pathology in children with lupus nephritis （LN）, analyzed the relationship between apoptosis and proliferation in LN. Methods Twenty - seven children （aged 7 - 16 years old, 21 type Ⅳ and 6 type Ⅴ ） with biopsy-proven LN and nine as controls were included in the study. Apoptosis was detected by in situ nick - end labeling techniques （TUNEL） in renal biopsy samples. Immunohistochemistry was employed to detect the proliferating cells identified by proliferating cell nuclear antigen （PCNA） and detect the expressions of proteins of apoptosis associated gene PDCD5 and Caspase - 3 in these patients. Results 1. Compared to type Ⅴ LN, the patients with type Ⅳ LN had more apoptotic cells,more PCNA positive cells and higher ratios of PCNA/apoptosis （P/A） in glomeruli. 2. There were no difference in expression of PDCD5 in glomeruli in type Ⅳ LN compared with those in type Ⅴ LN. Numbers of apoptotic cells were observed in glomeruli. The expression of Caspase - 3 in type Ⅳ LN increased in glomeruli compared with that in type Ⅴ LN. Conclusions The up - regulation of mechanism of apoptosis in type Ⅳ LN was less than that of type Ⅴ LN. Caspase - 3 participated in the apoptosis of glomeruli of LN, but PDCD5 did not play a role during apoptosis of glomeruli of LN or the effect of PDCD5 promoting apoptosis was depressed.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2005年第11期1090-1092,共3页
Journal of Applied Clinical Pediatrics
基金
北京人学人类疾病基金研究中心"985"计划项目资助(T-2)
