摘要
目的研究Carbogen长时间吸入的安全性,对肿瘤乏氧的改善以及对32P肿瘤间质内放疗疗效的影响。方法①荷S180肿瘤小鼠10只,行吸入Carbogen前、后的99mTcHL91SPECT乏氧显像,比较两者TNT的变化。②正常小鼠20只,分为对照组和Carbogen吸入组,后者每日吸Carbogen2h,共24d。观察小鼠的日常活动、体重变化,及重要脏器病理学变化。③荷S180肿瘤小鼠40只,分为未治疗空气组、未治疗Carbogen组、32P治疗空气组、32P治疗Carbogen组,观察、对比各组小鼠肿瘤生长情况,检测抑瘤率和抑瘤曲线。结果①Carbogen吸入前后肿瘤99mTcHL91的TNT分别为1.872±0.391和1.354±0.189,差异有统计学意义(t=4.476,P<0.01);②未治疗空气组、未治疗Carbogen组小鼠间肿瘤生长速率无明显区别;32P治疗空气组、32P治疗Carbogen组小鼠在治疗后第12天抑瘤率分别为66.01%和69.43%,两者间无明显区别,在治疗后第24天,肿瘤体积分别为(2.728±0.469)和(2.237±0.603)cm3(t=2.128,P<0.05),肿瘤质量分别为(2.437±0.447)和(1.965±0.538)g(t=2.134,P<0.05)。③正常小鼠Carbogen长期吸入后日常活动、体重变化等与正常对照组间无明显区别,心、脑、肺、肝、脾、肾、小肠等脏器未见明显异常病理学改变。结论Carbogen可以明显改善肿瘤乏氧状况,中长期吸入对小鼠毒副作用较小,在一定程度上可以增加32P肿瘤间质内放疗的疗效。
Objective To study the safety and improvement in tumor hypoxia and the ^32 P-colloid interstitial treatment effect with lasting earbogen-inhaling in murine tumor models. Methods 1. The ^99m Tc-HL91 SPECT imaging was performed in 10 S180 tumor-bearing mice before and after carbogen inhalation for 2 hours; 2. Twenty normal BALB/c mice were divided into an air-group and a carbogen-group, in which carbogen was inhaled by mice 2 hours per day for 24 days. The activity body weight were observed, and pathologic examination of important organs was taken; 3. Forty miee bearing S180 tumor were divided into four groups： untreated air-inhaling group, untreated carbogen-group, ^32 P-treated air-inhaling group, ^32 P-treated carbogeninhaling group. The volume of tumors and tumor inhibition curves were measured and compared in all mice, and the tumor inhibition ratio was calculated. Results 1. The TINT of ^99m Tc-HL91 before and after carbogen inhalation was 1.872 ±0.391 and 1.354 ± 0. 189, respectively （ t = 4.476, P 〈 0.01 ） ; 2. The tumor volume was not different between miee of untreated air-inhaling group and untreated earbogen-inhaling group; The tumor inhibition ratio was 66.01% and 69.43 % respectively in the mice of ^32 P-treated, air-inhaling group and ^32P-treated, earbogen-inhaling group on day 12 alter ^32 P-eolloid interstitial treatment, whereas on day 24 the tumor volume was 2.728±0.469 cm^3 and （2.237±0.603） cm^3（t = 2. 128, P〈0.05） and the tumor mass was 2.437 ± 0.447 and （ 1.965 ± 0.538）g （ t = 2. 134, P 〈 0.05） ; 3. There were no differences in the activity, body weight between untreated air-inhaling mice and untreated carbogen-inhaling mice. No abnormal pathologic ehanges of important organs were found in untreated earbogen inhalation miee. Conclusion Long-term carbogen inhalation could significantly improve tumor hypoxia and ^32 P-celloid interstitial treatment effect, and no toxicity was observed in mice.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2005年第5期437-440,共4页
Chinese Journal of Radiological Medicine and Protection
