摘要
目的:采用液相色谱质谱联用鉴定双参复方化学成分,同时应用均匀设计优化复方提取工艺。方法:按U6(6^2)均匀设计法提取双参复方,提取液经LCESIMS/MS检测分析,通过一级质谱和二级质谱鉴定复方中化学成分,并用提取离子流色谱(EIC)选取各成分的峰面积进行均匀设计软件处理,优选水提工艺的加水量和提取时间。结果:从双参复方中鉴定出8种人参皂苷成分和6种丹参水溶性成分。当加入25倍水,煮沸35min时,以上14种成分提取率最高,提取率均大于90。结论:为双参复方进一步开发利用提供了必要的研究基础。
AIM： To identify chemical components in Shuangshen Prescription by Liquid Chromatography-Mass Spectrometry （LC-MS）, and optimize the extraction by uniform design （UD）. METHODS： UD（U6（6^2）） was used to design the experiments of extraction, and LC-ESI-MS/MS was used to identify the components by MS^1 and MS^2. Meanwhile, we chose the extracted ion chromatography （EIC） area of each compound for applying UD analysis to optimize the factors influencing the extracration rate of Shuangshen Prescription, i.e. the amount of water added and the extracting time. RESULTS： Eight ginsenosides and six water-soluble components of salviae were identified in Shuangshen Prescription by LC/MS/MS. The extraction rate which was more than 90% for each of those 14 components, was highest under the condition of adding 25 times of water and extracting for 35 min. CONCLUSION： The study offered thc essential basis for further development and application of Shuangshen Prescription.
出处
《中成药》
CAS
CSCD
北大核心
2005年第10期1128-1132,共5页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(30271663
90209005)
十五攻关项目(2002BA906A293)资助
关键词
双参复方
均匀设计
液质联用
成分鉴定
提取工艺
Shuangshen Prescription
uniform Design
liquid chromatography-mass spectrometry （ LC-MS ）
component identification
extraction
