摘要
目的:建立以紫外分光光度法测定O/W型紫草脂肪乳剂中总萘醌含量的方法.方法:总萘醌的含量以左旋紫草素计,溶剂为甲醇,检测波长为516nm.结果:左旋紫草素检测浓度在8.32～41.60μg/ml范围内与吸收度呈良好的线性关系(r=1.0000,n=5),平均回收率为98.6%(RSD=0.53%).结论:本方法简便、准确,回收率与精密度高,重现性好,可用于O/W型紫草脂肪乳剂中总萘醌含量的测定.
OBJECTIVE： To establish a method for content determination of the total naphthoquinonc in oil/water emulsion of shikonin by ultraviolet spcctrophotomctry.METHODS： The content of the total naphlhoquinone was counted in L-shikonin,mcthanol was used as solvent and the wavelength was set at 516nm.RESULTS：The concentration of L shikonin had good linear correlation with absorbancc in the range of 8.32～41.60μg/ ml（r=1.0000, n= 5）, the average recovery was 98.6% （RSD= 0.53% ） . CONCLUSIONS： The present method is siraplc, precise and repllcablc resulting in high recovery and accuracy,it can be used to determine the content of total naphthoouinone in oil/wator cmulsion of shikonin.
出处
《中国药房》
CAS
CSCD
北大核心
2005年第20期1577-1578,共2页
China Pharmacy
关键词
O/W型
紫草脂肪乳剂
左旋紫草素
总萘醌
含量测定
Oil/water
Fat emulsion of shikonin
L- shikonin
Total naphthoqulnone
Content determination
