摘要
目的观察肠缺血/再灌注(I/R)致肺损伤时肺内HO-1/CO与iNOS/NO的相互作用。方法采用肠缺血/再灌注模型。32只Wistar大鼠随机分为假手术组(Sham组)、肠缺血1 h再灌注6 h组(I/R组)、氨基胍组(AG组)和血晶素组(hemin组)。检测肺组织中HO-1和iNOS的表达,观察肺组织丙二醛(MDA)、血清一氧化氮(NO)及动脉血中氧血红蛋白(Hb-CO)的含量,同时观察肺组织病理形态学改变。结果与Sham组比较,I/R组HO-1和iNOS表达显著增强(均P<0.01);AG组HO-1和iNOS表达较I/R组明显降低(均P<0.05);Hemin组iNOS表达较I/R组明显降低而HO-1表达明显升高(均P<0.05);I/R组肺组织MDA、血清NO、血中HbCO较Sham组显著增加(P<0.05或P<0.01);与I/R组比较,AG组、Hemin组肺组织MDA、血清NO显著降低(P<0.05或P<0.01)。AG组的HbCO明显降低而Hemin组的HbCO明显升高(P<0.05)。病理学检查显示,AG组与Hemin组肺组织损伤程度较I/R组明显减轻。结论NO及CO对肠I/R肺组织具有保护作用,两者之间存在着相互作用,肺内HO-1/CO的大量生成具有使NO产生减少的作用,同时iNOS/NO的过量生成具有上调HO-1表达使CO产生增多的作用。
Objective To observe the interaction between HO-1/CO and iNOS/NO in acute lung injury induced by intestinal ischemia - reperfusion in rats, Methods Thirty - two Wistar rats, weighed 200 ～ 250g were randomly divided into four groups with 8 animals in each group： sham - operation group, I/R group in which intestinal I/R was produced by clamping super mesenteric artery （SMA） for 1 hour and declamping SMA for 6 hours, AG group and Hemin group. MDA content in lung , the concentrations of CO in artery and the activities of serum NO were observed in the rat models of acute lung injury induced by intestinal ischemia - reperfusion. HO-1＇s and iNOS＇s expressions in rat lung tissue were detected by immunohistochemistry and morphometry computer image analysis. The histological changes of lung were observed under light microscope, Results Compared with sham group, HO-1＇s and iNOS＇s expressions were markedly increased in I/R group （P〈0.01）,But in AG group, HO-1＇s and iNOS＇s expressions were significantly lower than those in I/R group; in hemin group, expression of HO-1 was obviously higher while iNOS was significantly lower than that in I/R group（P〈0.05）. The concentrations of CO, content of MDA and the activity of serum NO in I/R group were significantly higher than those in sham group （P〈0.05 or P〈0.01）. The concentrations of CO was obviously higher in Hemin group, but was significantly lower in AG group（P〈0.05 or P〈0.01）. Under light microscope , the pathological changes induced by I/R were significantly attenuated by AG and Hemin. Conclusion Both CO and NO play a role in lung protection in intestinal ischemia- reperfusion, There is interaction between them, CO may in fluence iNOS/NO by way of HO-1 and inducement of iNOS may influence HO/CO.
出处
《中国急救医学》
CAS
CSCD
北大核心
2005年第10期728-730,共3页
Chinese Journal of Critical Care Medicine
基金
湖北省教育厅立项课题(No.2003×125)
关键词
肠
缺血/再灌注
急性肺损伤
血红素氧合酶
诱导型一氧化氮合酶
Intestine
Ischemia-reperfusion
Acute lung injury
Heme-oxygenase （HO-1）
Inducible nitric oxide synthase（iNOS）
