摘要
目的研究蛇毒抗高凝状态酶(AHCSE)对人肝癌细胞BEL-7404的作用及其可能的作用机制。方法应用相差显微镜、电子显微镜、MTT法、TUNEL法等方法观察BEL-7404细胞经过AHCSE处理后,细胞形态学、生物化学等方面的变化。结果人肝癌细胞BEL-7404经过AHCSE处理后,发生了形态学改变,胞浆内部分线粒体空泡化,成簇,胞膜下多见。浓度为0.5μg/ml的AHCSE对BEL-7404细胞即有很强的抑制作用,随着剂量的加大,其抑制率上升不明显。经AHCSE作用后,BEL-7404细胞发生了凋亡,凋亡率随AHCSE浓度的增加而增加。结论AHCSE对BEL-7404细胞具有很强的抑制作用,诱导细胞凋亡是其作用机制之一。
Objective To investigate the efficacy of antihypercoagulability state enzyme （AHCSE） on human hepatocellular carcinoma BEL-7404 cells and probe into its function. Methods MTT. Phase-contrast microscope, electron microscope, terminal deoxynudeotidy transferase dUTP nick and labeling （TUNEL） were conducted to observe the alteration of cell form and biochemistry variation of the AHCSEed BEL-7404 cells. Results The AHCSEed BEL-7404 cells altered in its forms. The low dosage AHCSE had a very strong inhibition to BEL-7404 cells, but with the dosage increase, the rate of inhibition did not rise markedly. After the action of AHCSE, Apoptosis took place in BEL-7404 cells, the apoptosis index rose with dosage increase. Conclusion The AHCSE has very strong inhibition on the BEL-7404 and inducing apoptosis is one of its functions.
出处
《皖南医学院学报》
CAS
2005年第4期245-248,共4页
Journal of Wannan Medical College
基金
安徽省教育厅自然科学研究基金项目(2004kj347)
