摘要
目的:建立以高效液相色谱法测定虎耳草中槲皮苷含量的方法。方法:色谱柱为C18(150mm×4.6mm,5μm),流动相为甲醇-0.2%H3PO4溶液(45∶55),流速为1.0ml/min,检测波长为350nm,柱温为室温。结果:槲皮苷进样量在0.40μg^2.00μg范围内线性关系良好(r=0.9996),加样回收率为95.33%(RSD=2.80%)。结论:本方法简便、稳定、快速、重现性好、无杂质干扰,可用于虎耳草质量控制。
OBJECTIVE： To establish a HPLC method for the determination of quercitrin in Saxifrage. METHODS： The analysis was performed on C18 column（150mm×4.6mm, 5μm）, the mobile phase was MeOH-0.2%H3PO4 （45 ： 55） with a flow rate at 1.0ml/min and wavelength at 350nm under room temperature. RESULTS： There was a good linear relationship when the sample size of quercitrin was at a range of 0.40μg～2.00μg（r = 0.9 996）, the recovery was 95.33% with RSD at 2.80%. CONCLUSION： This method was simple, stable, fast, and reproducible and without the interference of impurity, which can be used for the content determination of quercitrin in Saxifrage.
出处
《中国药房》
CAS
CSCD
北大核心
2005年第18期1418-1419,共2页
China Pharmacy
