摘要
目的探讨一次染汞的肾脏毒性作用并观察2氨基4(S丁基磺酰亚氨)丁酸(BSO)、还原型谷胱甘肽(GSH)、维生素C(VC)和二巯基丙磺酸钠(DMPS)预处理对汞肾脏毒性的影响。方法Wistar大鼠64只,随机分成8组。第1组为对照组,第2～4组为低、中、高剂量染汞组,分别皮下注射0.75、1.5和2.5mg kg的氯化汞溶液,第5～8组为预处理干预组。BSO预处理组先腹腔注射BSO0.5mmol kgbw,4h后皮下注射0.75mg kgHgCl2溶液。其他3个预处理组中,先分别腹腔注射GSH3mmol kg,VC4mmol kg或DMPS200μmol kgbw,2h后皮下注射2.5mg kgHgCl2溶液。注射容量均为5ml kgbw。对照组皮下注射生理盐水。注射12h后收集大鼠12h尿液,采集血液,分离血清,切取肝脏和肾皮质样品。测定肝脏、肾皮质和尿中汞含量;尿NAG、ALP、LDH活性和尿蛋白,血清尿素氮(BUN)含量。结果染汞后肝、肾皮质和尿汞含量随染汞剂量加大而逐渐增加。肾皮质汞含量有明显的剂量-效应关系,高剂量组肝汞含量显著高于中低剂量组和对照组。中高剂量组尿汞含量显著高于对照组。BSO预处理组和单纯0.75mg kgHgCl2组比,使肝汞含量增加,肾皮质和尿汞含量降低。GSH、VC和DMPS预处理组肝汞含量显著低于单纯2.5mg kgHgCl2组。尿NAG、ALP、LDH活性和尿蛋白、BUN含量随染汞剂量加大而升高,且2.5mg kgHgCl2组显著高于对照组、0.75和1.5mg kg HgCl2组。BSO预处理组尿NAG、ALP活性和尿蛋白、BUN含量显著高于单纯0.75mg kgHgCl2组和对照组。GSH、VC和DMPS预处理组和单纯2.5mg kgHgCl2组相比,尿NAG、ALP、LDH活性和尿蛋白、BUN含量显著降低。结论随着染汞剂量增加,肝脏、肾皮质和尿汞含量也增加。BSO预处理可增强汞的肾脏毒性作用,而GSH、VC和DMPS预处理则对汞的肾脏毒性具有一定的拮抗作用。
Objectlve To study the renal toxicity caused by mercury administrated once and to observe the effects of buthionine sulfoximine（BSO）, gluthionein （GSH）, vitamin C （ VC ）, and sodium 2,3-dimercato- 1-propanesulfonate（DMPS） pretreatment on the nephrotoxicity of mercury, Methods Sixty-four Wistar rats were divided randomly into eight groups, i, e,, control group, low, middle and high dose mercury groups and BSO, GSH, VC, DMPS pretreatment groups, The low, middle, and high dose mercury group rats were subcutaneously （sc） injected with 0,75, 1,5, and 2.5mg/kg HgCl2 , respectively. The BSO pretreatment group rats were intraperitoneally （ip） injected with 0.5mmol/kg BSO and four hours later sc administrated with 0.75mg/kg HgCl2, The GSH, VC and DMPS pretreatment group rats were ip injected with 3mmol/kg GSH, 4mmol/kg VC, 200μmol/kg DMPS, respectively, and two hours later sc administrated with 2,5mg/kg HgCl2 , The control group rats were sc injected with saline at corresponding time, The volume of injection was 5ml/kg body weight, The 12 h urine samples were collected after 12 hours, After 48 hours, the blood samples were collected and then centrifuged to get the serum. The liver and renal cortex were also removed, Mercury contents in the liver, renal cortex, and urine samples were measured, Urinary NAG, ALP, LDH activities, urinary protein and BUN contents were also determined, Results Mercury concentrations in the liver, renal cortex, and urine samples increased with mercury dose increasing, Mercury contents in the renal cortex presented evident dose-effect relationship, Mercury concentrations in the liver of high-dose mereury group were higher significandv than that of low. middle-dose mercury group, and control group. The concentrations of urinary mercury in the middle and high dose mercury groups were higher significantly than that of control group. Compared with 0.75mg/kg HgC12 alone group, BSO pretreatment increased mercury concentrations in the liver, but decreased the concentrations in t
出处
《卫生研究》
CAS
CSCD
北大核心
2005年第5期533-536,共4页
Journal of Hygiene Research
基金
辽宁省自然科学基金项目(No.962328)
