摘要
目的:自体角膜缘干细胞培养后自体移植被认为是眼表疾病的最佳治疗方法,因此,有必要深入认识角膜缘干细胞分子生物学的特征、组织学解剖和微环境以及自体角膜缘干细胞移植方面的研究进展。资料来源:应用计算机检索Medline1995-01/2002-12的文章,检索词“limbuscorneae,stemcells,autotransplant”,限定文章语言种类为Eng-lish;同时检索http://www.wanfangdata.com.cn1995-01/2002-12的文章,检索词“角膜缘干细胞,自体移植”,限定文章语言种类为中文。资料选择:选择与角膜缘干细胞组织学、生理病理学研究的相关及自体角膜缘干细胞培养移植相关文献进行初审。纳入标准:①动物实验研究。②临床观察应用研究。排除标准:①重复研究。②综述文献。资料提炼:共收集到120多篇上述相关文献。选择符合标准的15篇文章进行综合分析。资料综合:①角膜缘干细胞分子生物学的特征:角膜上皮干细胞具有其他定向干细胞相似的特性,有很强的增殖和细胞分裂能力;处于低分化状态;细胞周期长;分裂不对称。②角膜缘干细胞组织学解剖:在角膜缘基底部的Vogt栅栏区乳头状结构中的某些基底细胞即是角膜缘干细胞。③角膜缘干细胞与微环境:干细胞的增生和分化受到血清来源的因素及多种细胞因子的调控,因此,微环境中因素的变化直接影响着角膜缘干细胞的增生分化,从而导致各种角膜损伤性疾病的发生。④自体角膜缘干细胞移植:将严重眼表疾病患者健眼的自体角膜缘干细胞培养到羊膜载体上进行自体患眼眼表移植。观察术后2～5d内角膜上皮即可再生,1个月后视觉灵敏度明显增加,术后观察4个月,可见角膜上皮完整性以及持续性新生血管消退。结论:自体角膜缘干细胞培养后移植尚处于实验研究阶段,其中自体角膜缘干细胞培养后移植治疗眼表疾病已初步获得成功,然而这种移植术后
OBJECTIVE： Autotransplantation with cuhured autologous limbal stem cell is viewed as the best treatment. Therefore, it is necessary to further recognize the molecular biological character of limbal stem cell and histological anatomy and to study the progression of transplantation of autologous limbal stem cell. DATA SOURCES： The papers of Medline January 1995 to December 2002 were looked up in computer by retrieved words “limbus cornea,stem eells,autotransplant” of English version. Simultaneously, the papers of January 1995 to January 2002 of Chinese version were looked up by the same retrieved words in Chinese on http：//www.wanfangdata.com.cn. STUDY SELECTION： It is to examine initially the papers relevant with studies on histology and physiopathology of limbal stem cells and relevant with autotransplantation of cuhured limbal stem cells. Inclusion criteria： ①Animal experimental research②Research on clinical observation and application. Exclusion criteria： ① Repeated researches②Summarized literature. DATA EXTRACTION： More than 120 papers of relevant literatures were collected in total and 15 papers of those met the criteria and selected for synthetic analysis. DATA SYNTHESIS： ① Molecular biological characters of limbal stem cells： Stem cells of corneal epithelium present similar characters as other committed stem cells, with very strong capacity of proliferation and cell division, in poor differentiation state, long cellular cycle and unparallel division. ②Histological anatomy of limbal stem cells： Some basal cells in papillary structure of limbal basal palisades of Vogt are limbal stem cells. ③Limbal stem cells and microenvironment： Since proliferation and division of stem cells are controlled by serum-source factors and various kinds of cellular factors, the changes in factors in microenvironment affect directly the proliferation and division of limbal stem cells and result in various corneal injuries.④ Autotransplantation of limbal stem cells：Autologous limbal stem
出处
《中国临床康复》
CSCD
北大核心
2005年第30期263-265,共3页
Chinese Journal of Clinical Rehabilitation
