摘要
目的:利用组织工程技术体外重建角膜上皮组织,为重建角膜表面提供良好的移植材料和方法。方法:以等量凝血酶和纤维蛋白原作用生成的纤维蛋白胶为载体,分别种植新生兔和成兔角膜缘组织块,体外培养重建角膜上皮层,观察两组细胞的生长覆盖面积,t检验比较两组细胞的生长差异,并从生长特性、形态学特点及免疫细胞化学方面进行观察检测和比较。结果:两组兔角膜缘上皮细胞在纤维蛋白胶上黏附生长增殖,生长覆盖面积差异无统计学意义。体外培养7天左右即能较好地形成单层上皮细胞,2周左右形成的复层上皮细胞与纤维蛋白胶构成角膜上皮组织经检测与生理状态下的角膜上皮组织相近。结论:以纤维蛋白胶为载体、新生兔和成兔体外培养的角膜上皮组织片有些可作为眼表重建角膜上皮移植良好的来源之一。
Objective:To reconstruct corneal epithelial tissue in vitro by tissue engineer technique to provide corneal epithelial transplantation materials and methods for corneal surface reconstruction. Methods:Newborn and adult rabbit corneal limbal epithelial and primary cells were seeded on fibrin glue which were produced by equivalence Thrombin and Fibrinogen. The composite corneal epithelia cultured in vitro were examined by light and electron microscopy, immunocytechemical behavior. The two groups’ percentage coverage of cell growth was recorded every day and compared with independed sample t test. Results:The limbal epithelial cells of the two groups could be cultured and proliferated on fibrin glue without significant differences. Single layer cells were formed after 7 days and 3-4 layers epithelia were formed after 10-14 days. The compound epithelial tissue was composed of fibrin glue and stratified epithelial cells, which expressed epithelial cytokeratin(ck1). The cultured corneal epithelia were quite similar to the normal epithelial layers in vivo. Conclusion:The corneal epithelia cultured from newborn rabbit or adult rabbit cultured on the fibrin glue are proved to be potential grafts for ocular transplantion.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第5期304-306,F002,共4页
Journal of Nanjing Medical University(Natural Sciences)
关键词
角膜上皮
细胞培养
纤维蛋白胶
新生兔
corneal epithelia
cell culture
fibrin glue
newborn rabbit
