摘要
目的:比较根管治疗期间炎症急性发作及慢性根尖周炎患牙根管内8种厌氧菌检出情况,分析急性发作时根管内定植细菌种类及其相关性。方法:分别提取26例根管预备后约诊期间炎症急性发作患牙根管样本和23例慢性根尖周炎患牙根管样本,提取样本细菌DNA,利用细菌16S rRNA引物通过PCR扩增方法鉴定细菌。结果:慢性根尖周炎样本根管细菌检出率达100%(23/23),根管治疗期间急性发作样本细菌检出率为92.31%(24/26);产黑普氏菌、齿垢密螺旋体和直肠弯曲杆菌在急性发作样本中的检出率较慢性根尖周炎样本显著增高(P<0.05)。急性发作样本中牙龈卟啉菌与福赛氏类杆菌的检出显著相关(OR>2,P<0.05)。结论:根管内感染是根管治疗期间炎症急性发作的重要原因,厌氧菌在急性发作的发生中起重要作用。
AIM：To dcleet 8 kinds of microorganisms in the root eanals of teeth suffering from endodontic interappointment flare -up compared with those in the root canals of chronie apieal periodontitis, and to investigate the eombinations of baeteria found in flare -up cases. METHODS： Clinieal samples were collected from 26 teeth suffering from endodontie flare -up after eanal preparation and 23 infeeted teeth associated with ehronie apieal periodontitis. A 16S rRNA -based polymerase ehain reaetion （PCR） method was used in this study to identify 8 relative bacterial pathogens in the root eanals .DNA was extraeted from the samples and amplified respectively using universal 16S rRNA printers and the 8 baeterial speeifie 16S rRNA primers.RESULTS：100%（23/23） of chronie apical periodontitis samples and 92.31%（24/26） of endodontie flare -up samples were positive for the presence of bacteria. In samples of endodoutic flare -up.the deteeting rates of Prerotella nigrescens, Treponema denticola and Campylobacter recms were significantly increased in comparing with samples of ehroaic apical peridodontitis （P〈0.05） and there was a synergistic relationship between Bacteroides forsytlms and Porphyromonas gingiralis in the root eanal of endodontic interappointment emergeney cases （P〈0.05）.CONCLUSION： Microbial infeetion,especially anaerobe,appeared to play an important role on the occureme of endodontic interappointmant flare -up.
出处
《牙体牙髓牙周病学杂志》
CAS
2005年第9期500-503,共4页
Chinese Journal of Conservative Dentistry
基金
上海市教委资助课题(03BZ05)
上海市口腔医学重点实验室(03650513)
"十五"国家科技攻关计划(2004BA720A23)
关键词
根管治疗期间急性发作
PCR
根管
endodontic flare -up
polymerase ehain reaction （PCR）
root canals
