摘要
目的探讨胎膜早破时NF-κB在胎膜的表达和母血中IL-15的水平。方法免疫组化方法检测30例胎膜早破(PROM)和30例非PROM孕妇胎膜中NF-κB P65亚单位的表达,ELISA法检测两组孕妇血清IL-15含量。结果PROM组胎膜NF-κB P65亚单位阳性细胞数和IL-15在母血中含量高于非PROM孕妇(P<0.001)。PROM组胎膜NF-κB P65亚单位阳性细胞数和母血中IL-15含量随着破膜时间延长明显升高(P<0.05)。PROM绒毛膜羊膜炎组胎膜中NF-κBP65亚单位阳性细胞数和母血中IL-15含量高于非绒毛膜羊膜炎组(P<0.001)。结论PROM中胎膜NF-κB活性升高和孕妇血清中IL-15含量升高,PROM伴绒毛膜羊膜炎时二者亦升高。
Objective To evaluate the clinical significance of expression of NF-kB and the role of IL-15 in premature rupture of the fetal membranes（PROM）. Methods Using immunohistological method to test the quantity of NF-kB in the specimens of 30 PROM patients and 30 non-PROM patients. Using ELISA to test the quantity of IL- l5 in pregnancy woman＇ s sera. Results The percentage of NF-kB P65 positive cells in fetal membrane and the level of IL-15 in maternal blood in PROM patients were significantly higher than those in non-PROM patients （P 〈 0. 001 ）. The percentage of NF-kB P65 positive cells in fetal membrane and the level of IL-15 in maternal sera in PROM patients were increased obviously with the prolongation of time of PROM. The percentage of NF-kB P65 positive cells in fetal membranes and level of IL-15 in maternal sera in PROM patients with chorioamnionitis were significantly higher than those in PROM patients without chorioamnionitis. Conclusions The NF-kB activity in fetal membrane and IL-15 level in maternal sera in PROM are increased, both are increased in PROM with chorioaminonitis.
出处
《解剖科学进展》
CAS
2005年第3期216-219,共4页
Progress of Anatomical Sciences
