摘要
目的用基因重组技术将HIV-1 p24基因以及gp41基因具有抗原线性中和表位的部分重组连接,构建重组质粒,并在大肠埃希菌中高效表达融合蛋白。方法设计带有酶切位点的引物,分别PCR扩增p24和gp41两个基因,将它们分别连接到pMD18-T载体中,测序验证后,挑选出含有目的基因的正确克隆。将p24片段酶切后连接到gp41基因所在的pMD18-T载体中,再将连接后的两个基因酶切,重新连接到pET21a表达载体中。将表达载体转染大肠埃希菌诱导表达,经Western-blot验证表达正确。结果融合蛋白p24-gp41在大肠埃希菌中高效表达。结论融合蛋白p24-gp41可以在pET21a表达载体中高效表达。
Objective To construct recombinant plasmid with p24 and gp41 gene, express fusion protein in E. coli. Methods To design primer with restriction endonuclease position,and amplify p24 and gp41 by RT-PCR, link both into pMD18-T vector. To choose correct clone with target gene. Then p24 fragment will be cleaved and linked into pMD18 T vector within gp41 gene. Both post-linked gene will be cleaved and linked into pET21a vector. The vector will be transformed into E. coll. And protein is highly effective expressed in E coli. Western blotting proved that the expressed product could react with 6 × his antibody. Result Fusion protein p24-gp41 is highly effective expressed in E. eoli. Conclusion Fusion protein p24-gp41 is highly effective expressed in E. coli in pET21a vector.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2005年第3期170-173,共4页
Chinese Journal of Infectious Diseases
基金
教育部归国人员基金资助(教育部26)